动态监测血清VEGF在宫颈鳞癌化疗前后的变化

目的研究宫颈癌化疗前后血管内皮生长因子（vascular endothelial growth factor，VEGF）的改变，探讨VEGF能否作为评判化疗近期疗效的预测指标，为制定合理的治疗方案提供参考。方法采用酶联免疫吸附法（ELlSA）检测60例宫颈鳞癌患者及20例正常健康妇女血清中VEGF水平并进行对照分析。结果宫颈癌患者血清中VEGF水平显著高于正常健康妇女血清中VEGF水平（P〈0．01），且宫颈癌Ⅰ期血清VEGF值低于Ⅱ期者（P〈0．05）；宫颈癌患者术后血清VEGF表达较术前明显上升（P〈0．01），术后化疗后血清VEGF水平较术后明显下降（P〈0．01）；宫颈癌Ⅰ期患者术后化疗后血清VEGF水平与术前比较差异无统计学意义（P〉0．05），宫颈癌Ⅱ。期、Ⅱ。期患者术后化疗后血清VEGF水平均低于术前（P〈0．05）；宫颈癌Ⅱ。期患者新辅助化疗（neoadjuvant chemotherapy，NACT）后血清VEGF浓度较NACT前降低（P〈0．05）。结论VEGF可作为预测宫颈癌化疗近期疗效和判断术后是否需要辅助化疗及化疗疗程数的参考指标。

Dynamic monitoring of the changes of serum VEGF in cervical squamous cell carcinoma before and after chemotherapy

Objective To explore the feasibility of applying the vascular endothelial growth factor （VEGF） to predicting and evaluating cervical cancer chemotherapy by investigating the changes of VEGF before and after chemotherapy, so as to guide the rational treatment. Methods Using enzyme - linked immunosorbent assay（ELISA） to detect and compare serum VEGF levels in 60 cases of cervical squamous cell carcinoma and in 20 healthy women. Results Levels of serum VEGF in cervical carcinoma were significantly higher than those of normal healthy women（P 〈0. 01 ） ,and the serum VEGF values of cervical cancer in Stage Ⅰ were lower than those in Stage Ⅱ（P 〈 0. 05）. Expressions of serum VEGF in cervical cancer patients significantly increased after surgery compared with that before surgery（ P 〈0.01 ）, and decreased significantly after postoperative chemotherapy compared with that after surgery（P 〈0. 01 ） ,but the serum VEGF levels in Stage I cervical cancer patients after postoperative chemotherapy showed no significant difference compared with that before surgery（P 〉 0.05 ）, while those in Stage Ⅱ a and Ⅱ b cervical cancer patients after postoperative chemotherapy were lower than those before surgery（ P 〈0.05）. The expressions of serum VEGF in Stage Ⅱ b cervical cancer patients after neoadjuvant chemotherapy（NACT） were lower than those before NACT（ P 〈 0. 05 ）. Conclusion VEGF can be used as the reference for forecasting chemotherapy effects in cervical cancer and for determining whether to reinforce chemotherapy and postoperative chemotherapy regimen.