| 广州地区尖锐湿疣患者人乳头瘤病毒检测与基因分型 |
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| 引用本文： | 冯琼,肖青,邱秀. 广州地区尖锐湿疣患者人乳头瘤病毒检测与基因分型[J]. 中国热带医学, 2010, 10(2): 142-143,152 |
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| 作者姓名： | 冯琼 肖青 邱秀 |
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| 作者单位： | 广州市妇女儿童医疗中心,广东,广州,510180 |
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| 基金项目： | 中国癌症基金会资助的“中国生殖器疣的多中心临床流行病学研究”的华南地区子课题 |
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| 摘 要： | 目的分析广州地区尖锐湿疣(Condylomata acuminata,CA)患者人乳头瘤病毒(HPV)感染的型别特点,为临床防治提供参考依据。方法采用"中国生殖器疣的多中心临床流行病学研究"课题组所制定的标准检测技术———通用引物聚合酶链反应(PCR)及基于序列分析的基因分型方法,检测HPV型别。结果经PCR扩增分析153份CA患者活检标本中,93例HPVDNA阳性(60.8%);PCR阳性标本中,75例成功分型;单一型别感染率为47.1%,复合型别感染率为2.0%;高危型HPV16检出24例(15.7%),低危型HPV6和HPV11型分别检出27例(17.6%)和24例(15.7%)。结论本地区CA患者HPV感染以单一型别及HPV6、HPV11和HPV16型为主;感染高峰集中于40岁以下的性活跃期人群。
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| 关 键 词： | 尖锐湿疣 人乳头瘤病毒 型别分析 活检标本 |
Detection of human papilloma virus in condyloma acuminata outpatients and genetic typing in Guangzhou area |
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| FENG Qiong,XIAO Qing,QIU Xiu. Detection of human papilloma virus in condyloma acuminata outpatients and genetic typing in Guangzhou area[J]. China Tropical Medicine, 2010, 10(2): 142-143,152 |
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| Authors: | FENG Qiong XIAO Qing QIU Xiu |
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| Affiliation: | 1. Guangzhou Municipal Women and Child Health Care Center,Guangzhou 510180, Guangdong P.R. China) |
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| Abstract: | Objective To detect and genetically type multiple human papilloma virus(HPV)from condyloma acuminata outpatients and genetic typing in Guangzhou area. Methods Standard detection techniques-PCR,RFLP and nucleotide sequencing were were used to detect and identify HPV genotypes from individual lesions. Results After PCR amplification,93 of 153 samples were positive for HPV DNA . The positive rates of single and multiple HPV types were 47.1% and 2.0%,respectively. The infection rate of high risk HPV16 genotype ... |
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| Keywords: | Condylomata acuminata Human papilloma virus Typing analysis Biopsy samples |
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