Calpain activation and CaMKIV reduction in spinal cords from hSOD1G93A mouse model |
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| Institution: | 1. Unitat de Senyalització Neuronal, Dept Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, IRBLLEIDA, Rovira Roure, 80, 25198 Lleida, Spain;2. Dept Medicina Experimental, Facultat de Medicina, Universitat de Lleida, IRBLLEIDA, Rovira Roure, 80, 25198 Lleida, Spain;1. Department of Neurology, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, Hebei Province, China;2. Department of Clinical Medicine, Tangshan Vocational and Technical College, Tangshan 063000, Hebei Province, China;3. Department of Internal Medicine, Tangshan Union Medical College Hospital, Tangshan 063000, Hebei Province, China;4. Department of Neurosurgery, The Second People''s Hospital of Liaocheng, Liaocheng 252600, Shandong province, China;5. College of Psychology, North China University of Science and Technology, Tangshan 063000, Hebei Province, China;6. School of Public Health, North China University of Science and Technology, Tangshan 063000, Hebei Province, China;7. Institute of Mental Health, North China University of Science and Technology, Tangshan 063000, Hebei Province, China |
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| Abstract: | Amyotrophic Lateral Sclerosis (ALS), a severe neurodegenerative disease, affects the upper and lower motor neurons in the brain and spinal cord. In some studies, ALS disease progression has been associated with an increase in calcium-dependent degeneration processes. Motoneurons are specifically vulnerable to sustained membrane depolarization and excessive elevation of intracellular calcium concentration. The present study analyzed intracellular events in embryonic motoneurons and adult spinal cords of the hSOD1G93A ALS mouse model. We observed activation of calpain, a calcium-dependent cysteine protease that degrades a variety of substrates, and a reduction in calcium–calmodulin dependent protein kinase type IV (CaMKIV) levels in protein extracts from spinal cords obtained at several time-points of hSOD1G93A mice disease progression. However, in cultured embryonic motoneurons these differences between controls and hSOD1G93A mutants are not evident. Our results support the hypothesis that age-dependent changes in calcium homeostasis and resulting events, e.g., calpain activation and CaMKIV processing, are involved in ALS pathogenesis. |
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