Inflammation of the liver causes mutations in duck hepatitis B virus genome |
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Authors: | Ryo Fukuda MD Naruaki Kohge Shuji Akagi Nguten Thanh Xuan Alejandro Tokuda Shiro Fukumoto |
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Institution: | 1. Second Department of Internal Medicine, Shimane Medical University, 89-1 Enya-Cho, IzumoShi, 693, Shimane, Japan
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Abstract: | To investigate whether hepatitis causes mutation in the viral genome, DNA sequences in the pre-core region of duck hepatitis
B virus (DHBV) DNA were analyzed in both ducks with hepatitis and without hepatitis. Five DHBV carrier ducks were injected
with DHBV particle proteins purified from duck serum with Freund’s complete adjuvant (FCA) intrahepatically from 14 day posthatch
for 9 weeks (immunized group). Serum was drawn at the end of the 1st and 4th week after the 1st injection of DHBV particle
protein and ducks were killed at the end of the 9th week to obtain the liver. Another five ducks without treatment were used
as controls. All ducks of the immunized group showed moderate to severe hepatitis at the 9th week. All ducks in the immunized
group showed one mutation except one duck that showed two mutations only at the 9th week. Mutations were observed in the 5th,
13th, 21st, 22nd, and 28th codon of the precore region. All of them were point mutation at the 3rd base in the triplets. The
frequency of mutation was different in each duck from 20% to 60% but not 100%. There was no mutations in ducks in control
group. These results suggest that hepatitis causes mutation in the pre-core lesion genome of duck hepatitis B virus. |
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Keywords: | duck hepatitis B virus pre-core mutation polymerase chain reaction |
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