用NucliSens HIV-1 QT试剂盒定量测定精浆中的HIV-1 RNA

目的探讨NucliSens HIV-1 QT试剂盒用于定量测定HIV-1感染者人群精液或精浆中HIV-1的可行性。方法在正常人精液、精浆和血浆中分别添加5个滴度的HIV-1RNA,用NucliSens HIV-1QT测定,观察精液和精浆成分对测定结果有无影响,进一步测定、分析15名HIV-1感染者血浆和精浆中的HIV-1病毒载量。结果发现精液中因含有严重抑制核酸扩增的现象故不能使用NucliSens HIV-1QT,但精浆中未见该抑制现象。用Nu-cliSens HIV-1QT测定分别添加HIV-1RNA的正常人血浆和精浆样本,结果之间差异无统计学意义;所测定的10份正常人精浆样本,未发现假阳性结果。在15名HIV-1感染者中,血浆和精浆的HIV-1检出率分别为80%(12/15)和40%(6/15),病毒载量范围分别为(<250-140000)cp/ml和(<250-46000)cp/ml。结论NucliSens HIV-1QT可用于定量测定HIV-1感染者人群精浆中的HIV-1,精浆和血浆中HIV-1病毒载量之间具有一定的相关性。

Quantitative measurement of human immunodeficiency virus type 1 RNA in seminal plasma with Nuclisens HIV-1 QT

Objective To evaluate NucliSens HIV-1 QT(bioMerieux,Netherlands) in quantitating human immunodeficiency virus type 1(HIV-1) RNA in whole semen or seminal plasma from HIV-1-infected people.Methods Five levels of HIV-1 RNA were spiked to whole semen,seminal plasma and blood plasma samples from healthy people,and then measured by NucliSens HIV-1 QT.The same method was used to measure HIV-1 RNA in seminal plasma and blood plasma samples from 15 HIV-1 infected people.Results Nucleic acid amplification inhibitors were found in whole semen but not in seminal plasma when using NucliSens HIV-1 QT.No significant difference was found between normal seminal plasma and blood plasma samples spiked with HIV-1 RNA,and no false positive result was found in 10 normal seminal plasma samples.For 15 cases of HIV-1 infection,HIV-1 was detected in 80%(12/15) of the plasma samples and 40%(6/15) of the seminal plasma samples,with viral loads of <250 to 140,000 cp/ml and <250 to 46,000 cp/ml,respectively.Conclusions NucliSens HIV-1 QT could be used for quantitation of HIV-1 RNA in seminal plasma from HIV-1-infected people in China,and the viral loads in seminal plasma were correlated with those in blood plasma