P21WA F_1/CIP_1和P53基因表达与膀胱癌生物学行为的关系

目的 :探讨膀胱癌 P2 1WAF1 / CIP1 、P5 3基因的表达、相互间的调控及其与生物学行为的关系。方法 :采用免疫组织化学 ABC方法检测 12 2例有随访结果的膀胱癌患者的 P2 1WAF1 / CIP1 、P5 3基因表达 ,并采用双变量相关分析、L ogistic回归分析和 Kaplan- Meier法分析。结果 :P5 3野生型肿瘤中 P2 1WA F1 / CIP1 阳性表达率明显高于 P5 3突变型肿瘤 ;P5 3突变型肿瘤中保持 P2 1WAF1 / CIP1 阳性表达者具有与 P5 3野生型肿瘤同样低的复发率和较长的生存期。结论 :维持 P2 1WAF1 / CIP1 的表达可清除 P5 3突变蛋白的有害作用 ;根据 P2 1WAF1 /CIP1 表达状况 ,结合临床分期可对膀胱癌的恶性程度及预后作出更准确的评估 ,对拟定治疗方案有重要的指导意义

Expression and interaciton of P21WAF_1/CIP_1 and P53 genes in human bladder carcinoma and the clinical significance

Purpose:To investigate the correlation between the expression and interaction of P21WAF1/CIP1 and P53 genes in bladder carcinoma and clinical prognosis.Methods:In 122 cases of bladder cancer,the expression of P21WAF1/CIP1 and P53 were assessed by immunohistochemical techniques. The results were analyzed using the double variable quantity, Logistic return and Kaplan-Meier methods.Results:Among the P53 wild-type tumors,78% were P21WAF1/CIP1 positive compared with 41% of the P53-altered tumors (P＜0.01＝;Patients with P53-altered tumors that maintained P21WAF1/CIP1 expression showed the similar rates of recurrence and overall survival compared with patients with P53 wild-type tmors.Conclusions:Maintenance of P21WAF1/CIP1 expression appears to abrogate the deleterious effects of P53 alteration on bladder cancer progression; the status of P21WAF1/CIP1 expression considering together clinical stage can more accurately judge the malignance and prognosis for bladder cancer. Then the result has a significant indicator for working out therapy scheme.