Cell-type specific messenger functions of extracellular calcium in the anterior pituitary. |
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Authors: | Dragoslava Zivadinovic Melanija Tomi? Davy Yuan Stanko S Stojilkovic |
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Institution: | Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-4510, USA. |
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Abstract: | Calcium can serve not only as an intracellular messenger, but also as an extracellular messenger controlling the gating properties of plasma membrane channels and acting as an agonist for G protein-coupled Ca(2+)-sensing receptors. Here we studied the potential extracellular messenger functions of this ion in anterior pituitary cells. Depletion and repletion of the extracellular Ca(2+) concentration (Ca(2+)]e) induced transient elevations in the intracellular Ca(2+) concentration (Ca(2+)]i), and elevations in Ca(2+)]e above physiological levels decreased Ca(2+)]i in somatotrophs and lactotrophs, but not in gonadotrophs. The amplitudes and duration of Ca(2+)]i responses depended on the Ca(2+)]e and its rate of change, which resulted exclusively from modulation of spontaneous voltage-gated Ca(2+) influx. Changes in Ca(2+)]e also affected GH and PRL secretion. The PRL secretory profiles paralleled the Ca(2+)]i profiles in lactotrophs, whereas GH secretion was also stimulated by Ca(2+)]e independently of the status of voltage-gated Ca(2+) influx. Ca(2+)]e modulated GH secretion in a dose-dependent manner, with EC(50) values of 0.75 and 2.25 mM and minimum secretion at about 1.5 mM. In a parallel experiment, cAMP accumulation progressively increased with elevation of Ca(2+)]e, whereas inositol phosphate levels were not affected. These results indicate the cell type-specific role of Ca(2+)]e in the control of Ca(2+) signaling and secretion. |
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