| 口腔颌面部肿瘤细胞系中PTEN抑癌基因蛋白表达的研究 |
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| 引用本文： | 刘斌,吴军正,段小红,李洁,李焰. 口腔颌面部肿瘤细胞系中PTEN抑癌基因蛋白表达的研究[J]. 细胞与分子免疫学杂志, 2003, 19(1): 35-37 |
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| 作者姓名： | 刘斌 吴军正 段小红 李洁 李焰 |
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| 作者单位： | 第四军医大学口腔医学院口腔生物学教研室,陕西,西安,710032 |
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| 基金项目： | 第四军医大学创新工程课题资助(No.CX99A011) |
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| 摘 要： | 目的：研究口腔颌面部恶性肿瘤细胞系内源性PTEN蛋白的表达，比较高转移性癌细胞系与亲本细胞PTEN蛋白表达的差异。方法：应用ABC免疫组化染色法，检测8例癌细胞系PTEN蛋白的表达，包括舌癌细胞系Tca8113和HSC-3，口底癌细胞系HSC-2，颊癌细胞系BcaCD885，粘液表皮样癌细胞系MEC-1，腺样囊性癌细胞系SACC83以及高转移性舌癌细胞系Tb-TLP和高转移性粘液表皮样癌细胞系M3SP2。结果：5/8例癌细胞系PTEN蛋白的表达呈阳性，PTEN蛋白定位于靠近细胞核周围的细胞质中，3/8例癌细胞系PTEN蛋白的表达呈阴性，即BcaCD885细胞及高转移性Tb-TLP和M3SP2细胞。结论：PTEN蛋白表达缺失与癌细胞转移可能有定的关系。
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| 关 键 词： | 口腔颌面部肿瘤 细胞系 PTEN 抑癌基因 蛋白表达 |
| 文章编号： | 1007-8738(2003)01-035-03 |
| 修稿时间： | 2002-04-01 |
Expression of PTEN protein in oromaxillofacial tumor cell lines |
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| LIU Bin,WU Jun-zheng,DUAN Xiao-hong,LI Jie,LI Yan. Expression of PTEN protein in oromaxillofacial tumor cell lines[J]. Chinese journal of cellular and molecular immunology, 2003, 19(1): 35-37 |
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| Authors: | LIU Bin WU Jun-zheng DUAN Xiao-hong LI Jie LI Yan |
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| Affiliation: | Department of Oral Biology, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China. kqyljd@fmmu.edu.cn |
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| Abstract: | AIM: To investigate expression of PTEN protein in oromax-illofacial tumor cell lines and to compare the difference of PTEN protein expression between highly metastatic cancer cell lines and their parent cell lines. METHODS: ABC im-munohistochemical staining was applied for detecting expression of PTEN protein in 8 kinds of cancer cell lines, including tongue cancer cell lines Tca8113 and HSC-3, oral bottom cancer cell line HSC-2, buccal cancer cell line BcaCD885, mucoepidermoid carcinoma cell line MEC-1, adenoid cystic carcinoma cell line SACC83, highly metastatic tongue cancer cell line Tb-TLP and highly metastatic mucoepidermoid carcinoma cell line M3SP2. RESULTS: Of the 8 kinds of cancer cell lines, 5 kinds of cancer cell lines were positive for PTEN protein expression. PTEN protein was located in cytoplasm around nucleus. 3 kinds of cancer cell lines, BcaCD885, Tb-TLP and M3SP2, lacked expression of PTEN protein. CONCLUSION; Deficiency of PTEN protein expression may play a certain role in cancer cell metastasis. |
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| Keywords: | PTEN cancer cell line metastasis immunohis-tochemical staining |
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