| 熊果酸对人肝癌SMMC-7721细胞周期的影响 |
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| 引用本文： | 虞燕霞,顾振纶,殷江临,周文轩,郭次仪,梁中琴.熊果酸对人肝癌SMMC-7721细胞周期的影响[J].苏州大学学报(自然科学版),2011,31(1):71-74. |
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| 作者姓名： | 虞燕霞 顾振纶 殷江临 周文轩 郭次仪 梁中琴 |
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| 作者单位： | 虞燕霞 (苏州大学医学部,药理学系,江苏苏州,215123) ； 顾振纶 (苏州中药研究所,江苏苏州,215007) ； 殷江临 (苏州市立医院,药剂科,江苏苏州,215002) ； 周文轩 (苏州中药研究所,江苏苏州,215007) ； 郭次仪 (苏州市立医院,药剂科,江苏苏州,215002) ； 梁中琴 (苏州中药研究所,江苏苏州,215007) ； |
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| 基金项目： | 香港保健协会科研基金资助项目 |
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| 摘 要： | 目的探讨熊果酸（UA）对人肝癌SMMC-7721细胞周期的影响。方法用不同浓度的UA处理SMMC-7721细胞,采用四甲基偶氮唑盐（MTT）法测定细胞增殖的抑制作用,流式细胞仪检测细胞周期的变化,RT-PCR检测细胞周期相关基因p21、p53及增殖细胞核抗原（PCNA）的表达。结果 UA对SMMC-7721细胞生长有显著的抑制作用,且此作用呈明显的时间、剂量依赖性;UA可以诱导SMMC-7721细胞阻滞在S期,RT-PCR结果显示其能够上调p21和p53基因,下调PCNA基因。结论 UA能明显抑制SMMC-7721细胞的生长,引起细胞周期阻滞;细胞周期阻滞的发生与p21、p53、PCNA基因变化相关。
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| 关 键 词： | 熊果酸 肝癌 细胞周期 |
Effects of Ursolic Acid on Cell Cycle Regulation of Human Hepatoma Cell Line SMMC-7721 |
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| YU Yan-xia,GU Zhen-lun,YIN Jiang-lin,ZHOU Wen-xuan,GUO Ci-yi .LIANG Zhong-qin.Effects of Ursolic Acid on Cell Cycle Regulation of Human Hepatoma Cell Line SMMC-7721[J].Suzhou University Journal of Medical Science,2011,31(1):71-74. |
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| Authors: | YU Yan-xia GU Zhen-lun YIN Jiang-lin ZHOU Wen-xuan GUO Ci-yi LIANG Zhong-qin |
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| Institution: | 1.Dept of Pharmacology,Medical College,Soochow University,Jiangsu Suzhou 215123,China; 2.Suzhou Institute of Chinese Materia Medica,Jiangsu Suzhou 215007,China; 3.Dept of Pharmacy,Suzhou Municipal Hospital,Jiangsu Suzhou 215002,China) |
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| Abstract: | Objective To investigate effect of ursolic acid on cell cycle regulation of human hepatoma cell line SMMC-7721.Methods After treatment with UA,the growth inhibition of SMMC-7721 cells was assessed by MTT colorimetric assay.The effect of UA on the cell cycle phase distribution of SMMC- 7721 cells was analyzed using flow cytometry.The gene expression of p21,p53 and PCNA was evaluated by RT-PCR.Results The proliferation of SMMC-7721 cells was significantly inhibited in a dose-and time-dependent manner after UA treatment.Flow cytometric analysis showed SMMC-7721 cells treated with UA were accumulated in S phase in the cell cycle.The genes expression of p21 and p53 was increased in SMMC-772 cells cultured with UA,while the gene expression of PCNA was decreased.Conclusion UA has significant inhibition effect on the growth of SMMC-7721 cells.It can block the cell cycle in the S phase.Cell cycle arrest is related with the expressions of p21,p53 and PCNA. |
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| Keywords: | ursolic acid hepatoma cell cycle |
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