| 高效毛细管电泳结合PCR法鉴别南葶苈子与车前子 |
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| 引用本文： | 郭丹,陈娜娜. 高效毛细管电泳结合PCR法鉴别南葶苈子与车前子[J]. 中国药房, 2010, 0(3): 232-233 |
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| 作者姓名： | 郭丹 陈娜娜 |
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| 作者单位： | 南方医科大学南方医院药学部;南方医科大学药学院; |
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| 基金项目： | 广东省医学科研课题(A2008376); 南方医科大学南方医院院长基金课题(20060019) |
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| 摘 要： | 目的:建立以高效毛细管电泳结合聚合酶链式反应(PCR)鉴别南葶苈子与车前子的方法。方法:采用毛细管(60cm×75μm),运行缓冲液为30mmol·L-1四硼酸钠(pH9.2),高压进样5s,分离电压为12kV,检测波长为254nm,分析南葶苈子与车前子的PCR扩增产物。结果:高效毛细管电泳能快速、准确地分离南葶苈子与车前子的PCR扩增产物,二者的电泳色谱图存在较大差异,容易区分。结论:本法简单、快捷、灵敏,可用于鉴别南葶苈子与车前子。
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| 关 键 词： | 南葶苈子 车前子 高效毛细管电泳 聚合酶链式反应 鉴别 |
Identification of Descurainia sophia and Plantago asiatica by HPCE and PCR Assay |
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| GUO Dan CHEN Na-na. Identification of Descurainia sophia and Plantago asiatica by HPCE and PCR Assay[J]. China Pharmacy, 2010, 0(3): 232-233 |
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| Authors: | GUO Dan CHEN Na-na |
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| Affiliation: | GUO Dan(Dept. of Pharmacy,Nanfang Hospital of Southern Medical University,Guangzhou 510515,China) CHEN Na-na(College of Pharmacy,Southern Medical University,China) |
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| Abstract: | OBJECTIVE： To establish an HPCE and polymerase chain reaction （PCR） assay for identification of Descurainia sophia and Plantago asiatica.METHODS： PCR products of D. sophia and Rasiatica were analyzed by capillary tube （60cm×75μm）, taking 30 mmol·L^-1 sodium tetraborate decahydrate （pH 9.2） as run buffer. The injection sample was performed by high pressure of 5 s at a separation voltage of 12 kV and with UV detection wavelength of 254 nm. RESULTS ： PCR products of D. sophia and P. asiatica were separated by HPCE rapidly and accurately. The significant differences were noted in electrophoregram between PCR products of D. sophia and P. asiatica.CONCLUSION： This method is simple, rapid and sensitive for identification of D.sophia and P. asiatica. |
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| Keywords: | Descurainia sophia Plantago asiatica HPCE PCR Identification |
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