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| 人脐血造血干/祖细胞能在鼠肝中生长 | |
| 引用本文： | 周胜利,董政军,宋剑秋,栾佐,赵春华,阎文英,郭双风,曲伟红,宋道刚.人脐血造血干/祖细胞能在鼠肝中生长[J].中国实验血液学杂志,2002,10(5):391-394. |
| 作者姓名： | 周胜利 董政军 宋剑秋 栾佐 赵春华 阎文英 郭双风 曲伟红 宋道刚 |
| 作者单位： | 1. 山东脐血造血干细胞中心,济南,250002 2. 济南军区总医院动物中心,济南,250002 3. 海军总医院小儿科,北京,100037 |
| 摘 要： | 肝脏干／祖细胞的分化、再生以及它的生物特性是一个非常活跃的研究领域。本研究报告当人脐血细胞经静脉输注给肝脏受损伤的及免疫功能缺陷的小鼠后，采用流式细胞术和组织配型(HLA)的方法，结果发现，在输注9周后鼠肝脏细胞内还存在有人脐血细胞，即使在灌洗后肝脏细胞的贴壁细胞培养及细胞集落(CFU—GEMM)中，也可以发现人脐血细胞的存在。结论：证实了脐血造血干／祖细胞能长期在肝脏中生长。 |
| 关 键 词： | 脐血 造血干细胞 造血祖细胞 肝脏细胞 小鼠 人 胚胎干细胞 细胞治疗 |
| 修稿时间： | 2001年9月8日 |
Human Umbilical Cord Blood Hematopoietic Stem/Progenitor Cells Can Grow up in the Mouse Liver |
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| ZHOU Sheng Li,DONG Zheng Jun ,SONG Jian Qiu,LUAN Zuo ,ZHAO Chun Hua ,YAN Wen Ying,GUO Shuang Feng,QU Wei Hong,SONG Dao Gang.Human Umbilical Cord Blood Hematopoietic Stem/Progenitor Cells Can Grow up in the Mouse Liver[J].Journal of Experimental Hematology,2002,10(5):391-394. | |
| Authors: | ZHOU Sheng Li DONG Zheng Jun SONG Jian Qiu LUAN Zuo ZHAO Chun Hua YAN Wen Ying GUO Shuang Feng QU Wei Hong SONG Dao Gang |
| Institution: | Shandong Cord Blood Stem Cell Center, Jinan 250002, China. shengliz@public3.bta.net.cn |
| Abstract: | The biological characterization, differentiation and regeneration of hepatic stem/progenitor cells are the one of very active and interested fields. In this report, intravenous injection of human umbilical cord blood(HUCB) cells into the BALB/c nu and SCID mice, an animal model for transplantation and liver injury, was reported. Using of flow cytometry and tissue typing(HLA), it was found that the HUCB cells were survived in mouse liver for 9 weeks. After separation from perfused liver, HUCB cells were detected by hematopoietic colonies(CFU GEMM) in hepatocyte culture. It was concluded that the transplanted HUCB hematopoietic stem/progenitor cells can be survived in the liver over a long period of time. |
| Keywords: | umbilical cord blood hematopoietic stem/progenitor cell hepatocyte |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |