黄芪总苷对大鼠神经元缺氧/复氧损伤的影响

目的 探讨黄芪总苷（astragalosides, AST）对大鼠神经元缺氧/复氧损伤的影响。 方法 建立原代培养的大鼠海马神经元的缺氧/复氧模型。采用四唑蓝（MTT）法和乳酸脱氢酶（LDH）释放法检测细胞活力；检测细胞上清液中丙二醛（MDA）和一氧化氮（NO）含量、超氧化物歧化酶（SOD）活性；Hoechst染色和AnnexinV/PI双染流式细胞术检测细胞凋亡；采用激光共聚焦显微镜和Ca2+荧光探针Fluo-3/AM观察细胞内游离钙Ca2+]i。 结果 黄芪总苷可以提高缺氧/复氧后神经元的活力，降低细胞上清液中MDA和NO含量，提高SOD活性，降低细胞凋亡率，减轻神经元内的钙超载。 结论 黄芪总苷对缺氧/复氧损伤的神经元具有保护作用，其机制可能与抗氧化和减轻钙超载有关。

Protective Effect of Astragalosides on Anoxia/Reoxygenation Injury of Hippocampal Neuron

Objective To study the effect of astragalosides（AST） on the anoxia/reoxygenation（A/R） injured neuron in rat.Methods Primary cultured rat’s hippocampal neurons were made into A/R model cells.The cell viability was detected by MTT assay and lactate dehydrogenase releasing methods;the activity of superoxide dismutase（SOD）,and contents of malondialdehyde（MDA） and nitride oxide（NO） in culture supernate were detected;the apoptosis rate of hippocampal neurons after A/R was measured by flow cytometry with double-staining of hoechst33258 and AnnexinVPI;and intracellular calcium ion Ca2＋]i was observed with a cofocal laser-scanning microscope and determined by fluorescent probe Fluo-3/AM.Results AST enhanced the cell viability of neurons after A/R injury,increased SOD activity and decreased the MDA and NO contents in supernate,reduced the A/R-induced apoptosis and decreased the calcium overload in neurons.Conclusion AST has the protective effects on A/R injured neurons,the mechanism is possibly related with its anti-oxidation and calcium overload reducing actions.