促血管生成素在大肠癌组织的表达及其临床病理意义

目的　观察促血管生成素（Ａｎｇ）-１、Ａｎｇ-２及其受体Ｔｉｅ-２在大肠癌中的表达，探讨其与大肠癌血管生成的关系及临床病理意义。方法　采用免疫组化ＰＶ-９０００二步法观察５０例大肠癌和１０例正常大肠黏膜组织中Ａｎｇ-１、Ａｎｇ-２、Ｔｉｅ-２的表达，采用ＣＤ１０５标记检测微血管密度（ＭＶＤ）。结果　大肠癌组织Ａｎｇ-１的表达低于正常大肠黏膜，Ａｎｇ-２、Ｔｉｅ-２的表达高于正常大肠黏膜，差异均有统计学意义（Ｐ＜０.０５）；Ａｎｇ-１、Ａｎｇ-２的表达在不同分化程度间差异有统计学意义（Ｐ＜０.０１）；Ａｎｇ-１在淋巴结转移组的表达低于无转移组（Ｐ＜０.０５）；Ａｎｇ-２在淋巴结转移组高于无转移组（Ｐ＜０.０５），在Ｄｕｋｅｓ'Ｃ、Ｄ期明显高于Ｄｕｋｅｓ'Ａ、Ｂ期（Ｐ＜０.０１）；Ｔｉｅ-２在淋巴结转移组的表达高于无转移组（Ｐ＜０.０５）；Ａｎｇ-２、Ｔｉｅ-２的表达与ＭＶＤ均呈正相关（ｒ＝０.３４５，Ｐ＜０.０５；ｒ＝０.２９９，Ｐ＜０.０５）。结论　促血管生成素与大肠癌的病理分化程度、Ｄｕｋｅｓ分期、淋巴结转移有关，并且在大肠癌血管生成过程中发挥重要作用。

Expression of angiopoietins in colorectal carcinoma and its clinicopathological significance

Objective To observe the expression of angiopoietin-1,-2 and its receptor Tie-2 in colorectal carcinoma,and to investigate the possible relationship between the expression of angiopoietins and the angiogenesis in colorectal carcinoma.Methods The expression and distribution of angiopoietin-1,-2 and their receptor Tie-2 in 50 colorectal carcinoma and 10 normal tissues were detected by immunohistochemical PV-9000 two steps method,and the microvessel density was determined by CD105 staining.Results Angiopoietin-1 expression was higher in normal colorectal tissues than that in colorectal carcinoma（P〈0.05）,but angiopoietin-2 and its receptor Tie-2 expression was higher in colorectal carcinoma than that in normal tissues（P〈0.05）.The expression of angiopoietin-1,-2 were different in various levels of differentiated colorectal carcinoma（P〈0.01）.Angiopoietin-1 expression was lower with lymph node metastasis than without lymph node metastasis（P〈0.05）,angiopoietin-2 expression was in reverse.Angiopoietin-2 expression was higher with lymph node metastasis than without lymph node metastasis（P〈0.05）.Expression of angiopoietin-2 was significantly higher in Dukes stage C＋D than that in Dukes stage A＋B（P〈0.01）.Tie-2 expression was higher with lymph node metastasis than without lymph node metastasis（P〈0.05）.Expressions of angiopoietin-2 and Tie-2 were positively correlated with MVD respectively（r=0.345,P〈0.05;r=0.299,P〈0.05）.Conclusion Angiopoietins have relationships with the differentiation stage,Dukes stages and lymph node metastasis of colorectal carcinoma,and play an important role in the angiogenesis of colorectal carcinoma.