| HPLC法测定莲房中槲皮素的含量 |
| |
| 引用本文： | 牛家丰,孔凡存.HPLC法测定莲房中槲皮素的含量[J].齐鲁药事,2012,31(4):205-206. |
| |
| 作者姓名： | 牛家丰 孔凡存 |
| |
| 作者单位： | 1. 枣庄市药品检验所,山东,枣庄,277102
2. 枣庄市立二院,山东,枣庄,277103 |
| |
| 摘 要： | 目的采用高效液相色谱法测定莲房中槲皮素的含量。方法色谱柱:Phenomenex-C18(4.6 mm×250 mm,5μm);流动相:甲醇-0.2%磷酸溶液(50∶50);检测波长:360 nm;流速:1 mL.min-1;柱温:30℃。结果槲皮素在0.204～2.040μg之间,线性关系良好,加样回收率99.44%,RSD=0.82%(n=6)。结论该方法操作简便、准确,具有很好的重现性和稳定性,可以用于莲房的质量控制。
|
| 关 键 词： | 高效液相色谱法 莲房 槲皮素 含量测定 |
Determination of quercetin in receptaculum nelumbinis by HPLC |
| |
| NIU Jia-feng
,
KONG Fan-cun.Determination of quercetin in receptaculum nelumbinis by HPLC[J].qilu pharmaceutical affairs,2012,31(4):205-206. |
| |
| Authors: | NIU Jia-feng KONG Fan-cun |
| |
| Institution: | 1. Zaozhuang Institute for Drug Control, Zaozhuang 277102, China 12. The Second Hospital of Zaozhuang , Zaozhuang 277103, China ) |
| |
| Abstract: | Objective To establish an HPLC method for the determination of quercetin in receptaculum nelumbinis. Methods The separation was performed on Phenomenex- C18 Column（4.6 mm × 250 mm,5 um） with methanol -0.2% phosphoric acid （50：50 ） as mobile phase. The flow rate was 1.0 mL . min -1 , the detection wavelength was 360 nm and the column temperature was 30 ℃. Results The linear range of quercetin was 0. 204 - 2. 040 ug （ r = 0. 999 7 ）, the average recovery was 99.44% and the RSD was 0.82% （ n = 6 ）. Conclusion This method was convenient, rapid and accurate with good reproducibility and stability, and it can be used for the quality control of receptaculum nelumbinis. |
| |
| Keywords: | HPLC Receptaculum nelumbinis Quercetin Determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
