uPAR反义RNA质粒对胶原型大鼠滑膜细胞外基质影响的初步研究

目的：初步探讨uPAR反义RNA表达质粒对CIA大鼠滑膜细胞外基质的影响。方法：50只雌性大鼠，其中10只为正常对照组，不做任何处理，其余40只建立Ⅱ型胶原诱导的大鼠类风湿性关节炎模型，分为4组，每组10只，为造模对照组、生理盐水治疗组、空质粒治疗组、重组质粒治疗组，后3组踝关节分别注射生理盐水、空质粒及uPAR反义RNA表达质粒，观察大鼠一般情况、关节炎指数，病理变化及免疫组织化学法检测踝关节滑膜uPAR、MMP-3的表达。结果：随着CIA免疫时间的延长，造模对照组、生理盐水治疗组、空质粒治疗组关节炎指数评分逐渐增加迅速，而重组质粒治疗组关节炎指数评分增加缓慢；与正常对照组比较，模型组大鼠滑膜，滑膜轻度增厚，滑膜新生血管开始增多，大量炎性细胞浸润、增生，免疫组织化学显示：模型对照组、生理盐水组及空质粒组之间无统计学意义（P〉0．05），模型对照组、生理盐水组、空质粒组与重组质粒治疗组之间差异有统计学意义（P〈0．01），MMP-3的表达正常大鼠与模型对照组比较，差异有统计学意义（P〈0．05），模型对照组与重组质粒治疗组之间差异有统计学意义（P〈0．01）。结论：uPAR反义RNA表达质粒能抑制细胞外基质的降解，为类风湿性关节炎的治疗提供理论依据。

The Primary Study of uPAR Antisense RNA Expressing Plasmid to the Synovial Extracellular Matrix of collagen-induced arthritis in Rats

Objective： To investigate the result of uPAR（ urokinase-type Plasminogen Activator Receptor ） antisense RNA expressing plasmid to the synovial extraeellular matrix （ECM）of collagen-induced arthritis in Rats. Methods ：50 female Wistar rats were divided into normal control group（ n = 10） and collagen-induced arthritis（CIA） group（ n =40）. The CIA rats was made by injecting in- tradermally chicken type-Ⅱ collagen After the CIA model was made successfully ,40 rats were randomly divided into 4 groups：10 as model control group, 10 as physiological saline treatment group by injecting physiological saline, 10 as vector plasmid treatment group by injecting pc DNA3.1 （-） and 10 as recombinant plasmid treatment group by injecting uPAR antisense RNA plasmid. All the rats were selected to observe the general condition , arthritis index ,pathological changes by H-E stained and the expression of uPAR and MMP-3 （Matrix Metalloproteina-3） detected by immunohistochemical method. Result：With the time lasting,the arthritis index of model control group,physiological saline treatment group and vector plasmid treatment group rose gradually and recombinant plasmid treatment group rose slowly;Comparing with the normal control group, there were synovium thickened lightly, synovium new vascrdar started to proliferate and inflammatory cells in synoviuar tissues in model controll group. There was no significant difference between model control group, physiological saline treatment group and vector plasmid treatment group （p 〉 0.05 ） and was significant difference between recombinant plasmid treatment group and other CIA groups in the expression of uPAR（ P 〈 0.01 ） ;There was no significant differenee between model control group ,physiological saline treatment group and vector plasmid treatment group（ p 〉0.05 ） and was signifieant difference between recombinant plasmid treatment group and model control group in the expression of MMP-3 （ P 〈 0.01 ）. Conclusion：The uPAR Antisense pl