| 大鼠肺微血管内皮细胞的培养及百草枯对其毒性作用研究 |
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| 引用本文： | 姜文,何庆. 大鼠肺微血管内皮细胞的培养及百草枯对其毒性作用研究[J]. 西部医学, 2010, 22(12): 2198-2200 |
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| 作者姓名： | 姜文 何庆 |
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| 作者单位： | [1]雅安市人民医院急诊科,四川雅安625000 [2]四川大学华西医院急诊医学科,四川成都610041 |
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| 摘 要： | 目的探讨大鼠肺微血管内皮细胞（PMVECs）的培养及百草枯（PQ）对大鼠PMVECs的毒性作用。方法雄性SD大鼠PMVECs的原代培养和鉴定。培养的大鼠PMVECs在不同浓度PQ的培养基中培养不同时间,通过MTT法、琼脂糖凝胶电泳来检测PQ对大鼠PMVECs的毒性作用。结果成功地培养出大鼠PMVECs。MTT法测试发现PQ对大鼠PMVECs的增殖代谢活性具有剂量和时间依赖性的抑制作用;琼脂糖凝胶电泳显示凋亡的DNA阶梯。结论 PQ在体外对大鼠PMVECs的增殖具有抑制作用并能诱导其凋亡;PMVECs凋亡可能是PQ所致的急性肺损伤的机制之一。
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| 关 键 词： | 肺微血管内皮细胞 百草枯 毒性 |
Direct toxic effects of paraquat (PQ) on PMVECs |
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| JIANG Wen,HE Qing. Direct toxic effects of paraquat (PQ) on PMVECs[J]. , 2010, 22(12): 2198-2200 |
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| Authors: | JIANG Wen HE Qing |
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| Affiliation: | 1.Department of Emergency,Ya＇an People＇s Hospital,Ya＇an 625000,Sichuan;2.Department of Emergency,West China Hospital,Sichuan university,Chengdu 610041,China) |
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| Abstract: | Objective To investigate the direct toxic effects of Paraquat（PQ） on PMVECs.Methods Cultured rat PMVECs were incubated with different concentrations of PQ for different time.The toxic effects were studied by MTT method and agarose gel electrophoresis.Results PQ inhibited the proliferation and metabolism of rat PMVECs in vitro.Conclusion PQ could inhibite the proliferative activity and induce apoptosis of rat PMVECs in vitro.Apoptosis of rat PMVECs may be one of the mechanisms of PQ induced acute lung injury. |
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| Keywords: | Pulmonary microvascular endothelial cells Paraquat |
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