Variations in mitochondrial DNA and gene transcription in freezing-tolerant larvae of Eurosta solidaginis (Diptera: Tephritidae) and Gynaephora groenlandica (Lepidoptera: Lymantriidae) |
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Authors: | Levin D B Danks H V Barber S A |
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Affiliation: | Department of Biology, University of Victoria, Victoria, B.C., Canada;;Biological Survey of Canada (Terrestrial Arthropods), Canadian Museum of Nature, Ottawa, Ontario, Canada |
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Abstract: | Respiration, mitochondrial (mt)DNA content, and mitochondrial-specific RNA expression in fat body cells from active and cold-adapted larvae of the goldenrod gall fly, Eurosta solidaginis, and the Arctic woolly bear caterpillar, Gynaephora groenlandica, were compared. Reduced amounts of mtDNA were observed in cold-adapted larvae of both E. solidaginis and G. groenlandica collected in fall or winter, compared with summer-collected larvae. mtDNA increased to levels similar to those of summer-collected larvae after incubation at 10 degrees C or 15 degrees C for 5 h. Mitochondrial-specific RNAs (COI and 16S) were observed in fat body cells of both active and cold-adapted E. solidaginis larvae. Our results suggest that mitochondrial proteins required for respiration may be restored rapidly from stable RNAs present in overwintering larvae. |
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Keywords: | goldenrod gall fly Arctic woolly bear caterpillar cold-hardiness freezing mitochondria mtDNA cytochrome oxidase 16S rRNA mRNA |
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