实验室检测脊髓灰质炎疫苗滴度应注意的问题

Ｈｅｐ－２细胞是脊髓灰质炎（脊灰）病毒的敏感细胞之一，其敏感性受多种因素制约，本文以培养Ｈｅｐ－２细胞是否使用乳蛋白水解物及小牛血清的质量差异对脊灰糖丸疫苗滴度的影响进行了分析。结果表明：乳蛋白水解物的使用可使脊灰三价糖丸疫苗总滴度平均提高０．３６ＬｏｇＣＣＩＤ５０／粒，显著高于未使用乳蛋白水解物培养的Ｈｅｐ－２细胞。另外，小中血清的影响不容忽视，本文发现有些进口小牛血清对病毒的生长产生非特异性抑制，对脊灰病毒的抑制滴度可达１．９ＬｏｇＣＣＩＤ５０／ｍｌ以上。这提示我们。组织培养试剂的更换应格外慎重，实验室标准体系的建立也是非常必要的。

Problems Concerned in Laboratory Test for Oral Poliomyelitis Vaccine Titer

Hep -2 cell is one of the sensitive cells for polio virus. Its sensitivity is affect- ed by various kinds of factors. The effect of Hep - 2 cell culture medium with or without lactalbumin hydrolysate and the quality of newborn bovine serum on the oral polio virus vac- cine titre was analyzed in this article. The result showed that the culture medium with lactalbumin hydrolysate could enhance the polio virus vaccine total titre by 0. 36 LogCCID50 /pill which was significantly higher than Hep - 2 cell culture without lactalbumin hydrolysate. Be- sides, the quality of newborn bovine serum should not be neglected. We discovered some im- ported newborn bovine serum had non - specific inhibition effect to the growth of polio virus . The inhibition titre to polio virus could reach above 1 . 9 Log CCI D50 /ml . It indicates that we should be more cautious when we need to change the tissue culture reagent. It' s also necessa- ry to build up a standard laboratory system.