光学比浊法与连续血小板计数法监测血小板聚集功能的比较

目的评价血小板功能检测仪PL-11应用的连续血小板计数方法 （PL-11）监测血小板功能的价值。方法通过光学比浊法（light transmittance aggregometry,LTA）与PL-11连续血小板计数法检测本院2012年26例服用氯吡格雷抗凝治疗的心血管病患者和45例健康志愿者的血小板聚集功能,分析两种方法相关性及差异。结果血小板聚集诱聚剂二磷酸腺苷（adenosine diphosphate,ADP）诱导的LTA与PL-11最大血小板聚集率（maximal aggregation ratio,MAR）存在较好相关性（r=0.766,P〈0.000 1）。分别用LTA与PL-11检测健康志愿者组、服药患者组,最大血小板聚集率均存在统计学差异（P〈0.000 1）。在两组人群中,LTA测得最大血小板聚集率范围均较PL-11广。PL-11在每例标本检测过程中,各测试点提供的平均血小板体积（mean platelet volume,MPV）变化趋势与检测期间血小板聚集率变化情况一致。结论 PL-11连续血小板计数法与＂金标准＂的光学比浊法检测血小板聚集功能时有较好的相关性,其应用价值可供临床及实验室参考。富血小板血浆标本与全血标本可能是两种方法检测结果差异的原因。

Platelet aggregation function monitored by light transmittance aggregometry and continuous platelet count

Objective To assess the value of continuous platelet count with platelet function analyzer PL-11 in monitoring platelet function.Methods Platelet function of 26 coronary artery disease（CAD） patients admitted to our hospital in 2012 for anticoagulant therapy with clopidogrel and 45 healthy volunteers was detected by light transmittance aggregometry（LTA） and continuous platelet count with platelet function analyzer PL-11,respectively.The correlation and the different results in the two methods were analyzed.Results The adenosine diphosphate（ADP）-induced maximal aggregation ratio（MAR） detected by LTA and continuous platelet count with PL-11 was well-correlated in CAD patients and healthy volunteers（r=0.766,P0.000 1）.The MAR detected by LTA was higher than that detected by continuous platelet count with PL-11 in CAD patients and healthy volunteers.The mean platelet volume（MPV） was consistent with the MAR in each sample measured by continuous platelet count with PL-11 at different time points.Conclusion The platelet function detected by continuous platelet count with PL-11 and LTA is similar,which can provide reference for their application in clinical practice and laboratory tests.Platelet-rich and whole blood samples may be the reason for the different results of the two methods.