Serotonin regulates osteoblast proliferation and function in
vitro
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| Authors: | SQ Dai LP Yu X Shi H Wu P Shao GY Yin YZ Wei |
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| Institution: | 1. Department of Orthopedic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China;2. Department of Obstetrics and Gynecology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China;3. Emergency Department, The First Affiliated Hospital, Soochow University, Suzhou, China |
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| Abstract: | The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter,
also has important functions outside the central nervous system. The objective of
this study was to investigate the role of 5-HT in the proliferation, differentiation,
and function of osteoblasts in vitro. We treated rat primary
calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and
assessed the rate of osteoblast proliferation, expression levels of
osteoblast-specific proteins and genes, and the ability to form mineralized nodules.
Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at
different stages of osteoblast differentiation. We found that 5-HT could inhibit
osteoblast proliferation, differentiation, and mineralization at low concentrations,
but this inhibitory effect was mitigated at relatively high concentrations. Six of
the 5-HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D,
5-HT2A, 5-HT2B, and 5-HT2C) were found to exist
in rat osteoblasts. Of these, 5-HT2A and 5-HT1B receptors had
the highest expression levels, at both early and late stages of differentiation. Our
results indicated that 5-HT can regulate osteoblast proliferation and function
in vitro. |
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| Keywords: | Serotonin Osteoblast Proliferation Differentiation 5-HT receptors 5-HT |
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