Immunogenicity and protective efficacy of clade 2.3.2.1c and clade 2.3.4.4c H5Nx avian influenza antigen bank vaccines in mice,Korea |
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| Institution: | 1. Avian Influenza Vaccine Research Laboratory, Avian Influenza Research and Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea;2. Pathologic Diagnostic Laboratory, Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea;1. Avian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea;2. College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea;1. Poultry Diagnostic and Research Center, University of Georgia, College of Veterinary Medicine, 953 College Station Rd, Athens, GA 30602 United States;2. Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, U.S. Dept. of Agriculture, Agricultural Research Service, 934 College Station Rd., Athens, GA, 30605, United States;1. Birds and Rabbit Medicine Department, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minoufiya, 22857, Egypt;2. Middle East for Vaccine Company (MEVAC?), Second Industrial Area, El-Salhyia El-Gededa, Sharkia, 44671, Egypt;3. Virology Department, Faculty of Veterinary Medicine, University of Sadat City Sadat City, Minoufiya, 22857, Egypt;4. Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), AHRI, ARC, 12818 Giza, Egypt;5. Poultry Diseases Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt;1. Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa;2. Western Cape Department of Agriculture, Veterinary Services, Muldersvlei Road, Provate Bag X1, Elsenburg, 7607, South Africa;3. South African Ostrich Business Chamber, Rademeyer Road, Oudtshoorn, 6220, South Africa |
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| Abstract: | - The immunogenicity and protective efficacy of inactivated clade 2.3.2.1c (rgKA435) and clade 2.3.4.4c (rgES2) H5Nx vaccines, which are representatives of an avian influenza antigen bank in Korea, were examined in mice. Mice were vaccinated twice and then challenged with homologous virus. Hemagglutinin inhibition and serum neutralizing antibody titers in the rgES2-vaccinated group were higher (4.4 ± 1.7 and 10.8 ± 2.3 log2, respectively) than those in the rgKA435-vaccinated group (2.8 ± 1.1 and 2.5 ± 0.9 log2, respectively). rgES2 conferred 100% protection, with no morbidity, no severe body weight loss, and no virus replication in any of the tissues tested. By contrast, 80% of mice in the rgKA435 group survived. One mouse in this group died at 10 dpi. Virus titers in the lung and turbinate were 102.5–3.5 TCID50/0.1 ml at 3–7 dpi and 101.5 TCID50/0.1 ml at 3–5 dpi, respectively. In particular, the viral titer in the turbinate from the rgKA435 group at 3 dpi was significantly lower than that in the equivalent control group (p < 0.05). The data suggest that both of these antigen bank vaccines are promising candidates for further evaluation in humans.
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| Keywords: | H5 Vaccine Immunogenicity Protective efficacy Mice Antigen bank |
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