Evaluation of the immune responses against reduced doses of Brucella abortus S19 (calfhood) vaccine in water buffaloes (Bubalus bubalis), India |
| |
| Affiliation: | 1. ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Ramagondanahalli, Yelahanka, Bengaluru 560 064, India;2. Intervet India Pvt. Ltd. MSD Animal Health, Briahanagar, Off Pune-Nagar Road, Wagholi, 412 207 Pune, India;3. Division of Animal Science, Indian Council of Agricultural Research, Krishi Bhawan, New Delhi 110 001, India;4. International Livestock Research Institute (ILRI), Block-C, First Floor, NASC Complex, CG Centre, DPS Marg, Pusa, New Delhi 110 012, India;1. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Buenos Aires, Argentina;2. CONICET-Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral (IDEHU), Buenos Aires, Argentina;1. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran;2. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;3. Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran;1. Genomic Bioinformatics Laboratory, Department of Molecular Biology, Ariel University, Ariel, Israel;2. Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel;3. Adelson School of Medicine, Ariel University, Israel |
| |
| Abstract: | BackgroundBrucella abortus S19 is the most widely used vaccine for the prevention of bovine brucellosis which remains the reference vaccine to which many other vaccine/s are compared. Considering the larger vaccination coverage by reduced dose of vaccine, the study aimed to compare reduced graded doses (1/10th, 1/20th and 1/100th) with standard dose of S19 vaccine (40 × 109CFU /dose) to determine the effective immunizing dose in water buffaloes.MethodsA total of 25 female buffalo calves (Bubalus bubalis) in the age group of 4–5 months were equally grouped into five animals each in four test and one control groups and given with specified vaccine dose. The blood samples were collected on post vaccination days 14, 28, 45, 60, 90 and 120 for assessing innate (TNF-α and IL-12), humoral (IgG antibodies against Brucella LPS) and cell mediated immune responses (IFN-γ, CD4 + and CD8 + counts).ResultsThe full dose, 1/10th and 1/20th reduced doses of S19 vaccine was capable of eliciting pathogen-specific antibody response, vaccine induced secretion of IL-12, TNF-α and IFN-γ with CD4 + and CD8 + effector T cell responses. Persistence of antibody and magnitude of immune responses were found dose dependent.ConclusionComparable immune responses were noticed with 1/10th reduced dose similar to standard dose. With this observation, decline of antibody titre will reduce the number of false positives and reduced dose of vaccine will facilitate larger vaccination coverage in the country. |
| |
| Keywords: | Buffalo Immune response India Reduced vaccine dose |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
