A ferritin nanoparticle vaccine for foot-and-mouth disease virus elicited partial protection in mice |
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| Affiliation: | 1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China;2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China;1. Animal Science Faculty, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogotá, Colombia;2. School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia;3. Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia;4. Dirección Técnica de Análisis y Diagnóstico Veterinario. Instituto Colombiano Agropecuario (ICA), Bogotá, Colombia;5. Faculty of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia;1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou, Gansu, China;2. College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China;3. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China;1. Biopharming Research Unit, Department of Molecular and cell Biology, University of Cape Town, Private Bag X3, Rondebosch, 7701, South Africa;2. Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory 7925, South Africa;3. Instituto de Virología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina |
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| Abstract: | Foot-and-mouth disease (FMD) is an acute, febrile, and highly contagious infectious disease common in cloven-hoofed animals. Outbreaks and epidemics of FMD can result in major economic losses of livestock. Using ferritin nanoparticles as the scaffold for an antigen can enhance the immunogenicity of the subunit vaccine and provide possible protection against FMD. We used a baculovirus expression system to express four recombinant proteins (VP1, VP1-Ft, G-H loop-Ft, and ferritin) and the protective immunity of the FMD ferritin nanoparticle vaccines was evaluated in mice. The recombinant subunit vaccines containing VP1, VP1-Ft, and G-H loop-Ft proteins significantly increased FMDV-specific IgG and IgG subclass antibody titers compared with the PBS group, as well as enhancing splenocyte proliferation and the expression of IL-4 and IFN-γ. The VP1 and VP1-Ft vaccines provided survival rates of 55.6% and 66.7%, respectively. The G-H loop-Ft vaccine provided a 77.8% survival rate compared with 100% survival in the inactivated vaccine group. The partial survival provided by the ferritin nanoparticle vaccines indicated that further study of the effects of the fused ferritin nanoparticle FMDV vaccines in animals is warranted. |
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| Keywords: | Foot-and-mouth disease Ferritin nanoparticle Recombinant Vaccines |
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