Immunogenicity of the Lyme disease antigen OspA,particleized by cobalt porphyrin-phospholipid liposomes |
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| Affiliation: | 1. Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and Environment (RIVM), Bilthoven, The Netherlands;2. Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands;3. Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands;1. Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands;2. Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands |
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| Abstract: | Outer surface protein A (OspA) is a Borrelia lipoprotein and an established Lyme disease vaccine target. Admixing non-lipidated, recombinant B. burgdorferi OspA with liposomes containing cobalt porphyrin-phospholipid (CoPoP) resulted in rapid, particulate surface display of the conformationally intact antigen. Particleization was serum-stable and led to enhanced antigen uptake in murine macrophages in vitro. Mouse immunization using CoPoP liposomes that also contained a synthetic monophosphoryl lipid A (PHAD) elicited a Th1-biased OspA antibody response with higher IgG production compared to other vaccine adjuvants. Antibodies were reactive with intact B. burgdorferi spirochetes and Borrelia lysates, and induced complement-mediated borreliacidal activity in vitro. One year after initial immunization, mice maintained high levels of circulating borreliacidal antibodies capable of blocking B. burgdorferi transmission from infected ticks to human blood in a feeding chamber. |
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| Keywords: | Liposomes Adjuvant Particle vaccine Lyme disease OspA |
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