Protective efficacy of recombinant bacterin vaccine against botulism in cattle |
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| Affiliation: | 1. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, CEP 96160-000, Brazil;2. Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, CEP 68740-970, Brazil;1. Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India;2. Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India;3. Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India;1. ANSES, Ploufragan—Plouzané Laboratory, Avian and Rabbit Epidemiology and Welfare Unit, Ploufragan, France;2. ANSES, Ploufragan—Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France;3. Université Européenne de Bretagne, France;4. GDS Bretagne, Ploufragan, France;5. ANSES, Food Safety Laboratory, Plateform Identypath, Maisons Alfort, France;1. Centro de Desenvolvimento Tecnológico e Biotecnologia, Universidade Federal de Pelotas, CEP 96010-900, Pelotas CP 354, RS, Brazil;2. Escola de Veterinária, Universidade Federal de Minas Gerais, CEP 30123-970, Belo Horizonte CP 567, MG, Brazil;1. Direction of Veterinary Services, 97300, Cayenne, French, Guyana;2. Direction Générale Outre-Mer, 75007, Paris, France;3. Hospital Center, 97300 Cayenne, 97300, Cayenne, French, Guyana;5. National Reference Center for Anaerobic Bacteria and Botulism, Pasteur Institute, 75015, Paris, France;1. Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul CEP 96160-000, Brazil;2. Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará CEP 68740-970, Brazil |
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| Abstract: | Botulism is a paralytic disease caused by the intoxication of neurotoxins produced by Clostridium botulinum. Among the seven immunologically distinct serotypes of neurotoxins (BoNTs A − G), serotypes C and D, or a chimeric fusion termed C/D or D/C, are responsible for animal botulism. The most effective way to prevent botulism in cattle is through vaccination; however, the commercially available vaccines produced by detoxification of native neurotoxins are time-consuming and hazardous. To overcome these drawbacks, a non-toxic recombinant vaccine was developed as an alternative. In this study, the recombinant protein vaccine was produced using an Escherichia coli cell-based system. The formaldehyde-inactivated E. coli is able to induce 7.45 ± 1.77 and 6.6 ± 1.28 IU/mL neutralizing mean titers against BoNTs C and D in cattle, respectively, determined by mouse neutralization bioassay, and was deemed protective by the Brazilian legislation. Moreover, when the levels of anti-BoNT/C and D were compared with those achieved by the recombinant purified vaccines, no significant statistical difference was observed. Cattle vaccinated with the commercial vaccine developed 1.33 and 3.33 IU/mL neutralizing mean titers against BoNT serotypes C and D, respectively. To the best of our knowledge, this study is the first report on recombinant E. coli bacterin vaccine against botulism. The vaccine was safe and effective in generating protective antibodies and, thus, represents an industry-friendly alternative for the prevention of cattle botulism. |
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| Keywords: | Botulinum neurotoxins Formaldehyde-inactivated recombinant vaccine |
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