C2基因对人胃癌细胞周期的影响

目的 探索新克隆的编码人蛋白翻译起始因子的全长基因（C2基因）对细胞周期的影响及与细胞凋亡的关系。方法 构建C2基因真核表达载体，转染人胃癌细胞系SGC7901，获得瞬时和稳定表达C2蛋白的人胃癌细胞株，应用流式细胞分析法检测C2蛋白在转染细胞上的表达和转染细胞细胞细胞周期的改变并在电镜下观察转染细胞超微结构的改变。结果 成功构建了C2基因真核表达载体；流式细胞分析法检测转染细胞C2蛋白表达率为32．3％，空白对照为0．9％；细胞周期检测提示瞬时和稳定转染了C2基因的细胞均发生凋亡；电镜下可见转染了C2基因的胃癌细胞发生凋亡。结论 C2基因转染可引起胃癌细胞发生凋亡，机制有待进一步研究。

Effect of C2 gene on humengastric cancer cell cycle

Objective To investigate the effect of a new cloned full length gene, C2 gene which encodes a translation initiation factor, on cell cycle and the relationship between C2gene and apoptosis. Methods Construct a eukaryotic vector carrying the full length of C2 gene, then transfected it into a gastric cell line-SGC7901 cells and gained cells expressing C2 protein transiently or stably. Test the expression of C2 protein and the change of cell cycle of the transfected cells using the flurescence activatied cell sorting(FACS) and the changes of their ultra structure electromicroscopically. Results The C2 gene eukaryotic expression vector was successfully constructed. FACS showed the C2 protein expression ratio of transfected cells was 32.3%, while the empty control group was 0.9%. The test of cell cycle showed that there was appeared apoptosis peak in transiently or stably transfected cells. The apoptosis cells can be seen electromicroscopically among the C2 transfected cells. Conclusions C2 gene transfection can lead SGC7901 cells to apoptosis, but the mechanism has to be further study.