番茄红素对慢性苯染毒小鼠抗氧化酶的影响

背景苯中毒主要对造血系统和神经系统造成损伤,导致DNA损伤,DNA加合物形成等.番茄红素是一种重要的类胡罗卜素,具有多种生物学作用,如抗氧化、抗肿瘤、诱导细胞间隙连接通讯等. 目的探讨番茄红素对慢性苯染毒所造成的氧化损伤的拮抗作用.设计随机对照的实验研究.地点和材料哈尔滨医科大学公共卫生学院动物室.选用纯种雄性健康昆明鼠30只,由哈尔滨医科大学附属第二医院动物中心提供.干预措施分为对照组、苯染毒组和番茄红素组,每组10只.对照组不染毒,苯染毒组和番茄红素组静式吸入苯蒸气,番茄红素组给予番茄红素,60 d后处死,取肝、脾、脑组织.主要观察指标测定肝、脾和脑中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛含量.结果苯染毒能够明显,降低组织匀浆中SOD活性肝、脾、脑分别为(12.57±1.94),(5.57±0.81),(8.28 ±2.25)μkat/g],GSH-Px活力肝、脾、脑分别为(0.38±0.05),(0.52 ±0.05),(0.10±0.01)mkat/g],升高丙二醛含量肝、脾、脑分别为(2.85 ±0.38),(3.58 ±0.53),(3.10±0.89)μmol/g].给予番茄红素后可使小鼠组织匀浆的SOD活性(μkat/g)升高(肝、脾、脑分别为16.08±2.55,6.43±1.28,9.03±1.52),GSH-Px活力(mkat/g)上升(肝、脾、脑分别为0.53 ±0.08,0.62 ±0.07,0.12 ±0.01),丙二醛含量(μmol/g)降低(肝、脾、脑分别为2.26±0.31,2.98±0.37,2.79 ±0.37),与苯染毒组相比,差异有显著性(q=4.00～8.04,P＜0 05).结论番茄红素能够减轻苯染毒引起的脂质过氧化,提高小鼠机体的抗氧化能力,增强抗氧化酶的活力.

Impact of lycopene on antioxidative enzymes in mice with chronic benzolism

BACKGROUND: Benzolism(benzene poisoning) mainly hurts hemopoietic system and nervous system, which would induce DNA injury, and DNA adducts formation, etc. Lycopene is an important carotenoid, which has many biological effects, such as, antioxidation, anti-carcinoma and induction linkage and communication of intercellular space, etc.OBJECTIVE: To discuss the antagonism of Lycopene in antioxidative system injury caused by chronic benzolism.DESIGN: A randomised controlled study SETTING and MATERIALS: The study was conducted in the animal laboratory of the Faculty of Public Health of Harbin Medical University. 30 pure bred male healthy Kunming mice were obtained from the Experimental Animal Center of Second Hospital Affihated to Harbin Medical University.INTERVENTIONS: Animals were randomly allocated into normal control group, benzolism group and Lycopene group with 10 each. Animals in control group were not poisoned, but animals in benzolism group and Lycopene group statically inhaled benzene vapour. Lycopene was administrated only in Lycopene group but not benzolism group. Animals were executed after 60 days to remove liver, spleen and brain tissues for the preparation of homogenates.MAIN OUTCOME MEASURES: The activities of Superoxide dismutase (SOD) and glutathiione peroxidase(GSH-Px) as well as malondialdehyde (MDA) content in liver homogenate, spleen homogenate and brain homogenate were assayed.RESULTS: Benzolism would significantly affect the activities of antioxidative enzymes by decreasing SOD activities (12.57±1.94), (5.57±0. 81 ), (8.28 ± 2.25) ukat/g] and GSH-Px activities (0.38 ±0.05),(0.52±0.05), (0.10±0.01) mkat/g] and elevating MDA contents (2.85±0.38), (3.58±0.53), (3.10±0.89)μmol/gl] in thehomogenates of liver, spleen and brain respectively. After the administration of Lycopene, the activities of SOD(μkat/g) increased(16.08±2.55),(6.43±1.28), (9.03±1.52)], the activities of GSH-Px(mkat/g) (0.53 ± 0.08), (0.62 ± 0.07), (0. 12 ± 0.01) ] elevated, and MDA contents (μmol/g)(2.26±0.31), (2.98±0.37), (2.79±0.37)]decreased in the homogenates of liver, spleen and brain respectively, which all had significant differences compared with that of benzolism group (q =4.00-8.04, P ＜0.05).CONCLUSION: Lycopene could relieve the lipid peroxidation(LPO) induced by benzolism to improve the ability of antioxidation in mice by enhancing the activities of antioxidative enzymes for the antagonism of the oxidative injury caused by benzolism.