| 检测人血浆中利福平及其主要代谢物25-去乙酰利福平浓度的高效液相色谱法 |
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| 引用本文： | 王基平,李群慧,何小兵,张蓉.检测人血浆中利福平及其主要代谢物25-去乙酰利福平浓度的高效液相色谱法[J].东南大学学报(医学版),1999(2). |
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| 作者姓名： | 王基平 李群慧 何小兵 张蓉 |
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| 作者单位： | 南京铁道医学院基础医学部 |
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| 摘 要： | 目的：建立检测人血浆中利福平及其主要代谢物２５－去乙酰利福平浓度的高效液相色谱法，并用于药代动力学研究。方法：所用色谱柱是ＺｏｒｂａｘＯＤＳ（５μｍ，４．６ｍｍ×２５０ｍｍ），采用梯度洗脱，流动相Ａ和Ｂ由甲醇、乙腈和０．１ｍｏｌ·Ｌ－１ＮａＨ２ＰＯ４组成（流动相Ａ三者的体积比为３５∶２５∶４０，Ｂ为５０∶２５∶２５），以利福贲丁作为内标物，检测波长为３３６ｎｍ。结果：血浆标准曲线为：利福平，ＣＲＭＰ＝６．９１８Ａｐ／Ａｉｎ－０．０６４，ｒ＝０．９９９９；２５－去乙酰利福平，ＣＤＥＳ＝１０．９１Ａｄ／Ａｉｎ－０．０２９，ｒ＝０．９９９７。两者从血浆提取的平均回收率分别为９１．８％和８４．７％。批内和批间ＲＳＤ均＜８．６％。结论：本法可用于药代动力学和临床研究。
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| 关 键 词： | 高效液相色谱法 利福平 25－去乙酰利福平 血浆 |
HighPerformance Liquid Chromatography Assay for Detection of Rifampicin and
25Desacetylrifampicin in Human Plasma |
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| Wang Jiping,Li Qunhui,He Xiaobing,Zhang
Rong.HighPerformance Liquid Chromatography Assay for Detection of Rifampicin and
25Desacetylrifampicin in Human Plasma[J].Journal of Southeast Univ: Medical Sci Ed,1999(2). |
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| Authors: | Wang Jiping Li Qunhui He Xiaobing Zhang
Rong |
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| Abstract: | bjective: The purpose of the study was to develope a method for determination of
Rifampicin and 25Desacetylrifampicin with highperformance liquid chromatography in human
plasma for pharmacokinetic research. Methods: A Zorbax ODS column and mobile phases that
were composed of methanol, acetontrile and 0.1molL-1 NaH2PO4,were used for setting an
ultraviolet detection (336nm). Rifapentine was chosen as an internal standard. Results:
CRMP=6.918 Ap/Ain -0.064,r=0.9999;CDES=10.91 Ad/Ain-0.029,r= 0.9997. RSDs of intrabatch
and interbatch assays were lower than 8.6%. The average recoveries of Rifampicin and
25Desacetylrifampicin from plasma were 91.8% and 84.7%,respectively. Conclusion: This
method could be used in pharmacokinetic and clinical studies. |
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| Keywords: | highperformance liquid chromatography rifampicin 25desacetylrifampicin plasma |
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