| 应用基因微矩阵芯片筛选胰腺癌的相关基因 |
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| 引用本文： | 张弘,周国雄,黄介飞,徐岷,江枫,魏群.应用基因微矩阵芯片筛选胰腺癌的相关基因[J].江苏医药,2007,33(1):8-9,F0003. |
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| 作者姓名： | 张弘 周国雄 黄介飞 徐岷 江枫 魏群 |
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| 作者单位： | 226001,南通大学附属医院消化病科 |
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| 摘 要： | 目的 应用基因微矩阵芯片筛选胰腺癌的相关基因.方法 按一步法提取胰腺正常组织及胰腺癌组织总RNA,以包含4096个DNA的基因微矩阵芯片对正常胰腺组织及胰腺癌基因表达谱进行分析.结果 胰腺癌与正常胰腺组织中表达差别有显著意义的基因共有949条,其中胰腺癌下调基因468条,上调基因381条,按其功能分类共有15类.结论 基因微矩阵芯片可有效筛选出胰腺癌的相关基因;胰腺癌的发生发展由多个类型的基因参与调控,而非单一因素所致.
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| 关 键 词： | 胰腺癌 基因 基因微矩阵芯片 应用 相关基因 微矩阵芯片 筛选 胰腺癌 gene screening cDNA microarray 因素 调控 类型 发展 发生 功能分类 上调基因 下调基因 意义 差别 基因表达谱 结果 |
| 文章编号： | 0253-3685（2007）01-08-02 |
| 修稿时间： | 2006-02-23 |
Application of cDNA microarray in screening pancreatic cancer-related gene |
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| ZHANG Hong, ZHOU Guoxiong, HUANG Jiefei,et al..Application of cDNA microarray in screening pancreatic cancer-related gene[J].Jiangsu Medical Journal,2007,33(1):8-9,F0003. |
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| Authors: | ZHANG Hong ZHOU Guoxiong HUANG Jiefei |
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| Institution: | Department of Gastroenterology, Affiliated Hospital, Nantong University, Nantong 226001, CHINA |
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| Abstract: | Objective To screen the pancreatic cancer-related gene by cDNA microarray. Methods Total RNA was isolated by one-step protocol from pancreatic cancer and normal pancreatic tissue, and purified to mRNA by Oligotex. The genes in pancreatic cancer and normal pancreatic tissue were analyzed by cDNA microarray with 4 096 human genes. Results There were 849 differentially expressed genes in pancreatic cancer and normal pancreatic tissue, of which 468 were down-regulated and 381 were up-regulated in pancreatic cancer. According to function, they were divided to 15 kinds. Conclusion cDNA microarray can be used effectively to screen the pancreatic cancer-related genes, which are not regulated by any single gene. Many different kinds of genes are involved in the initiation and development of pancreatic cancer. |
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| Keywords: | Pancreatic cancer Gene cDNA microarray |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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