| Expression of MMP-3 mRNA in hypertrophic scar fibroblasts and MMP-3 gene transfection |
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| 作者姓名： | 赵烨德 刘宁飞 何清濂 许国铭 |
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| 作者单位： | Zhao Yede;(Department of Plastic Surgery，Chinese PLA 411 Navy Hospital，Shanghai 200081，China)Liu Ningfei;He Qinglian;(Department of Plastic Surgery，Changzheng hospital，Shanghai 200003，China)Xu Gnoming;(Department of Gastroentrology，Changhai hospital |
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| 摘 要： | Hypertrophicscarring,whichoftencausescosmeticproblemsandfunctionaldisorders,isacommonclinicalproblemforpatientswhohavesurvivedextensivethermalinjury;itrepresentsthedermalequivalentoffibroproliferativedisorders.Histologically,hypertrophicscarringischaracterizedbyexcessiveaccumulationofextracellularmatrix(ECM)inthewoundllj.However,themechanismforexcessivescarformationhasstillnotwellelucidated.Someresearchershavesuggestedthattheexcessivecollagenaccumulationmightbeduetothetransformationofnormalfi…
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Expression of MMP-3 mRNA in hypertrophic scar fibroblasts andMMP-3 gene transfection |
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| Zhao Yede,.Expression of MMP-3 mRNA in hypertrophic scar fibroblasts and MMP-3 gene transfection[J].Journal of Medical Colleges of PLA(China),1998(4). |
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| Authors: | Zhao Yede |
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| Abstract: | To investigate the molecular mechanism of extracellular matrix overdeposition in hypertrophic scar tissues and to explore MMPs gene therapy for hypertrophic scar. Methods: Hypertrophic scarderived and normal skin-derived fibroblasts were cultured and a recombinant retrovirus vector containing MMP-3 gene was constructed and then transfected into hypertrophic scar fibroblasts. Expressive level of MMP-3 mRNA was detected by dot blotting, and the activity of MMPs was determined by DNP-peptide.Results: Lower expression of MMP-3 mRNA and fewer DNP-peptide hydrolyzed fragments were observed in hypertrophic scar-derived fibroblasts compared with normal skin-derived fibroblasts. Transfection of MMP-3gene into hypertrophic scar-derived fibroblasts could enhance the expression of MMP-3 mRNA (3. 4 fold)and the de novo capacity to hydrolyze DNP-peptide (2. 1 fold). Conclusion: Overdeposition of extracellular matrix in hypertrophic scar tissue was related to low expression of MMP-3 due to its down-degradation of extracellular matrix. MMP-3 gene transfection could be a better way to treat hypertrophic scars by degrading extracellular matrix. |
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| Keywords: | hypertrophic scar matrix metalloproteinase fibroblast extracellular matrix gene transfer |
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