空调病患者外周血细胞及T淋巴细胞亚群检测的意义

目的 探讨空调病患者外周血细胞及淋巴细胞亚群变化的特点及意义.方法 用血常规及流式细胞术分析36例发热空调病患者(发热组)及30例非发热患者(非发热组)外周血细胞及淋巴细胞亚群的变化,并以30例健康人(健康组)作为对照进行分析.结果(1)3组患者白细胞比较无明显差别(F=1.89,P=0.134);(2)发热组、非发热组、健康组中性粒细胞百分比分别为(68.13±5.67)％、(53.24±8.36)％、(61.25±6.31)％,发热组与非发热组、健康组比较中性粒百分比明显升高(t=6.64,9.13;P=0.018,0.027),非发热组与健康组比较无明显改变(P＞0.05);淋巴细胞百分比为(31.66±7.11)％、(43.67±8.17)％、(30.98±8.76)％,与发热组、健康组比较非发热组血淋巴细胞百分比明显升高(t=5.59,4.98;P=0.022,0.021);(3)CD4+百分比分别为(36.351±6.531)％、(42.676±5.169)％、(39.253±5.461)％,与非发热组比较发热组明显下降(t=1.28,P=0.039),与健康组比较非发热组CD4+细胞明显上升(t=3.80,P=0.048);CD8+百分比分别为(28.084±7.344)％、(21.186±6.331)％、(24.607±7.108)％,发热组较非发热组CD8+则明显升高(t=3.17,P=0.016);(4)CD4+/CD8+百分比分别为(1.629±0.554)％、(2.380±0.391)％、(2.079±0.659)％,与非发热组及健康组比较发热组明显下降(t=19.21,6.98;P＜0.001,0.006),非发热组较健康组则明显升高(t=8.68,P=0.031).结论 空调病患者淋巴细胞亚群及比率的变化提示空调病患者细胞免疫功能网络的失衡.

Significance of changes of peripheral blood cells and T-lymphocyte subgroups in patients with aircondition disease

Objective To discuss the metabolic characteristics and significance of peripheral blood cell and T lymphocyte subgroup in patients with air condition disease.Methods Thirty-six patients diagnosed as air condition diseases with fever and 30 patients diagnosed as non-fever patients enrolled this study as observation group(fever group and non-fever group respectively),and 30 health population were used as control.The changes of peripheral blood cell and T lymphocyte was measured using regular blood examination and flow cytometry.Results(1)No significant diffence on white blood cell was found between the three groups(F =1.89,P =0.134);(2)The percentage of neutrophils was(68.13 ±5.67)％,(53.24 ±8.36)％ and(61.25 ±6.31)％ respectively in the fever,non-fever and control group,respectively.Compared to the non-fever and control group,the percentage of neutrophils were significantly higher(t =6.64,9.13; P =0.018,0.027 respectively).No signiciant difference in the percentage of neutrophils was found between the non-fever group and control group(P =0.356,0.125).The percentage of lymphocyte percentage was(31.66 ±7.11)％,in the fever group,which was significantly higher than those of(43.67 ±8.17)％ and(30.98 ± 8.76)％ in the nonfever and control group(t =5.59,4.98 ; P =0.022,0.021 respectively).(3)The percentage of CD4 + was (36.351 ± 6.531)％,(42.676 ± 5.169)％ and(39.253 ± 5.461)％ in the fever,non-fever and control group,respectively.The percentage of CD4 + in the fever group was significantly lower than that in the non-fever group(t =1.28,P =0.039),whereas the percentage of CD4 + in the non-fever group was significantly higher than that in the control group(t =3.80,P =0.048).The percentage of CD8 + was(28.084 ± 7.344)％,(21.186 ± 6.331)％ and(24.607 ± 7.108)％,respectively.Compared to nonfever group,the percentage of CD8+ was significantly higher(t =3.17,P =0.016).(4)The ratio of CD4 +/CD8 + was(1.629 ±0.554)％、(2.380 ± 0.391)％,and(2.079 ± 0.659)％.Compared to the non-fever and control group,the ration in the fever group was siginificantly lower(t =19.21,P ＜ 0.001 ; t =6.98 ; P =0.006),whereas compared to the control group,the ratio in the non-fever group was significantly higher(t =8.68,P =0.031).Conclusion The changes of T subgroup show the unbalance immunity function network in patients with air condition disease.