| 缺氧脐带间充质干细胞对脐血CD34＋祖细胞免疫反应的影响 |
| |
| 引用本文： | 严晓晔,李彤,侯跃龙,张臣.缺氧脐带间充质干细胞对脐血CD34＋祖细胞免疫反应的影响[J].天津医科大学学报,2010,16(1):18-21. |
| |
| 作者姓名： | 严晓晔 李彤 侯跃龙 张臣 |
| |
| 作者单位： | 1. 天津医科大学三中心临床学院,天津,300170
2. 天津市第三中心医院 |
| |
| 摘 要： | 目的：探讨脐带来源的缺氧间充质干细胞（MSCs）是否能有效抑制脐血CD34＋祖细胞免疫排斥反应，影响反应过程中免疫调节细胞因子的分泌。方法：从脐带中分离、培养MSCs，观察其生长形态，流式细胞术检测其表面标志；将缺氧处理后的脐带MSCs加到脐血CD34＋祖细胞、外周血单个核细胞（PBMc）共培养体系中，96h后收集上清液，ELISA检测IFN-γ和IL-10水平。结栗：脐带MSCs不表达CD40、CD80、CD86、HLA—DR、CD34；缺氧的脐带MSCs能抑制共培养体系中淋巴细胞IFN-γ的分泌（与对照组比，P〈0．05），同时促进IL-10分泌（与对照组比，P〈0．05），但与非缺氧脐带MSCs组相比，IFN-γ的分泌量增多，IL-10的分泌量减少。结论：缺氧脐带MSCs能抑制脐血CD34＋祖细胞的免疫排斥反应，抑制IFN-γ的分泌，同时促进IL-10分泌，但与非缺氧脐带MSCs相比，免疫调节功能下调。
|
| 关 键 词： | 间充质干细胞 CD34＋祖细胞 脐血 细胞因子 缺氧 |
Effects of hypoxic umbilical cord-derived mesenchymal stem cells on the immune response of umbilical cord blood CD34~+ cells |
| |
| YAN Xiao-ye,LI Tong,HOU Yue-long,ZHANG Chen.Effects of hypoxic umbilical cord-derived mesenchymal stem cells on the immune response of umbilical cord blood CD34~+ cells[J].Journal of Tianjin Medical University,2010,16(1):18-21. |
| |
| Authors: | YAN Xiao-ye LI Tong HOU Yue-long ZHANG Chen |
| |
| Institution: | YAN Xiao-ye1,LI Tong2,HOU Yue-long1,ZHANG Chen1 (1.The Third Central Clinical College,Tianjin Medical University,Tianjin 300170,China,2.The Third Central Hospital of Tianjin) |
| |
| Abstract: | Objective:To investigate the effect of hypoxic umbilical cord-derived mesenchymal stem cells (UC-MSCs) on the immune responses of umbilical cord blood (UCB)CD34+ progenitor cells,and the secretion of immunomodulattory cytokines.Methods:UC-MSCs were isolated and cultured,the growth morphology were observed.Surface markers of UC-MSCs were detected by flow cytometry.The hypoxic and non-hypoxic UC-MSCs were added to the co-culture system of UCB CD34+ progenitor cells and peripheral blood mononuclear cells (PBMC... |
| |
| Keywords: | Mesenchymal stem cells CD34+ progenitor cells Umbilical blood Cytokine Hyoxia |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
