RG50864对PDGF诱导的PASMC DNA含量及增殖细胞核抗原表达的影响

目的：研究酪氨酸蛋白激酶抑制剂ＲＧ５０８６４对ＰＤＧＦ诱导的肺动脉平滑肌细胞（ＰＡＳＭＣ）ＤＮＡ含量及增殖细胞核抗原表达的影响。方法：应用原代培养的小牛肺动脉平滑肌细胞，用流式细胞仪分析ＲＧ５０８６４对ＰＤＧＦ诱导的ＰＡＳＭＣＤＮＡ百分含量及增殖细胞核抗原的变化。结果：与对照组相比，ＲＧ５０８６４处理组可显著地抑制ＰＤＧＦ诱导的ＰＡＳＭＣ增殖细胞核抗原表达的荧光强度及荧光指数（Ｐ＜０．００１），增加ＰＡＳＭＣ生长周期中的Ｇ０／Ｇ１期的ＤＮＡ百分含量，抑制Ｓ期及Ｇ２Ｍ期ＤＮＡ百分含量（Ｐ＜０．０１或Ｐ＜０．００１）。结论：ＲＧ５０８６４可显著地抑制ＰＤＧＦ诱导的ＰＡＳＭＣ增殖细胞核抗原表达，抑制ＰＡＳＭＣ周期Ｓ期及Ｇ２Ｍ期ＤＮＡ百分含量，阻止ＰＡＳＭＣ周期由Ｇ０／Ｇ１向ＤＮＡ合成的Ｓ期及Ｇ２Ｍ期转化。

The Effects of RG50864 on the Expression of the Percent Content of PDGF Induced PASMC DNA and Proliferation Cells Nuclear Antigen

The study was designed to explore the effects of RG50864 on DNA percent and proliferation cell nuclear antigen (PCNA) of PDGF induced pulmonary artery smooth muscle cells (PASMC).Methods:By primary culture of calf PASMC,the changes of RG50864 on DNA percent content and PCNA of PDGF induced PASMC were observed by using flow cytometry.Results:As compared with control group,fluorescence intensity and index of PDGF induced PASMC PCNA expression were significantly inhibited by RG50864 groups ( P<0.001 ),DNA percent content of G 0/G 1 phase was increased,however,which of S and G 2M phase was decreased in RG50864 groups ( P <0.1 or P<0 001 ).Conclusion:RG50864 could significantly inhibite the PCNA expression of PDGF induced PASMC,and block the converting of cells from G 0/G 1 phase to S phase of DNA synthesis.