血清HMGB1与胎膜早破的关系研究

目的检测未足月胎膜早破（PPROM）与足月胎膜早破（PROM）孕妇血清高迁移率蛋白-1（HMGB1）的浓度，探讨其与胎膜早破的关系及对PPROM患者绒毛膜羊膜炎的预测价值。方法选取PPROM患者55例，相同孕周健康孕妇40例；PROM患者40例，相同孕周健康孕妇40例，进行血清HMGB1检测，PROM及PPROM患者分娩后胎盘胎膜病理检查。结果PROM及PPROM组孕妇血清中HMGB1含量均高于相应对照组（t=10．15，9．92，P〈0．01）；但PROM及PPROM组孕妇血清中HMGB1含量比较，差异无统计学意义（t=0．65，P〉0．05）。PPROM组中存在组织学绒毛膜羊膜炎者比无绒毛膜羊膜炎者血清HMGB1含量明显升高（t=7．25，P〈0．05）。结论HMGB1在PPROM及孕PROM孕妇血清中浓度升高，对PPROM患者绒毛膜羊膜炎的预测有一定价值。

Relationship between maternal plasma HMGB1 and premature rupture of membranes

Objective To investigate the relationship between maternal plasma high mobility groupbox1 (HMGB1) and premature rupture of membranes(PROM) by detecting the concentration of HMGB1 in pregnant women,and to explore the significance of HMGB1 in the diagnosis of chorioamnion itis inpregnant women with PPROM.Methods The serum HMGB1 concentrations were measured in 55 pregnant women with PPROM and 40 healthy pregnant wonen at same pregnant term.40 pregnant women with PROM at term and 40 healthy pregnant women at same pregnant term,and Hematoxylin Eosin (HE) staining of placenta were performed after delivery of PPROM and PROM at term cases to observe the pathological changes of membranes of placenta.Results The plasma levers of HMGB 1 in pregnant women with PPROM and PROM at term were significantly higher than those in control group(t = 10.15,9.92 ,P ＜ 0.01) ,however,there was no significant difference in the levers of HMGB1 between pregnant women with PPROM and pregnant women with PROM (t = 0.65,P ＞ 0.05).Furthermore there was significant difference in the levers of HMGB1 between PPROM group with chorioam nionitis(t =7.25,P ＜ 0.05).Conclusion The HMGB1 concentration in pregnant women with PPROM or PROM at term is increased,but its diagnostic value for chorioam nionitis in patients with PPROM needs to be researched further.