中药配伍对血清阿魏酸临床生物利用度的影响

目的：研究中药配伍对方剂吸收入人体内成分生物利用率的影响。方法：健康自愿者一次口服川芎汤（1g/kg)，川芎芍药汽（2g/kg，两药之比为1：1），冠心Ⅱ叼汤（3g/kg，其中川芎为0．5g/kg)，后，用建立的HPLC法测定吸收入循环血中的阿魏酸（FA）血药浓度－时间曲线，用梯形法计算血药浓度一时间曲线下面积（AUC），然后比较AUC值，结果：3组的血药浓度时间曲线均为双峰现象。与川芎汤的AUC（15734．88±7737．97）ng/(min.ml)比较，川芎配伍芍药后AUC(5717.92±6124.63)ng/(min.ml)值明显降低（P＜0．01），而川芎配伍在冠心II号（川芎的剂量仅为川芎汤的50％）中的AUC（8072．00±4424．31）ng/(min.ml)相对不低，结论：冠心病Ⅱ号中药不影响FA的生物利用度，川芎芍药汽则影响明显。

Effect of Combination of Chinese Herbal Drugs on Clinical Bioavailability of Ferulic Acid in Serum

OBJECTIVE: To observe the effect of combination of Chinese herbal drugs on bioavailability of Ligusticum walichii (LW) derived ferulic acid (FA) in serum. METHODS: Healthy volunteers were given LW decoction (1 g/kg), LW combined with Paeonia veitchii (PV, 1:1) decoction (2 g/kg) and Coronary Heart No. 2 (CH-II, 3 g/kg, containing LW 0.5 g/kg) once respectively. The serum FA concentration-time (c-t) curve was performed by HPLC using the method established by the authors, and the area under that curve (AUC) was calculated and compared. RESULTS: The FA c-t curve in the three groups was double peak curve. As compared with the AUC in subjects who were administered LW decoction, the AUC in those taken LW + PV decoction was lower [(15734.88 +/- 7737.97) ng/(min.ml) vs (5717.92 +/- 6124.63) ng/(min.ml)], P < 0.05), while that in those taken CH-II was relatively higher [(8072.00 +/- 4424.31) ng/(min.ml), P > 0.05)]. CONCLUSION: Combination of LW and PV could significantly lower the bioavailability of FA, but when LW was added in CH-II, the FA bioavailability was not affected.