乙酰半胱氨酸对小鼠肺成纤维细胞系3T3增殖及胶原合成的影响

目的 研究乙酰半胱氨酸(NAC)对小鼠肺成纤维细胞3T3增殖、凋亡和胶原合成的影响.方法 培养小鼠肺成纤维细胞3T3,不同浓度NAC处理后,MTT检测细胞增殖,流式细胞仪检测细胞凋亡率、细胞周期,RT-PCR检测Ⅰ型前胶原mRNA表达的变化.结果 ①5、10、20、40 mmol/L NAC对3T3细胞生长均有明显抑制作用,且呈剂量依赖性;②5、10、20、40 mmol/L NAC作用24 h后,细胞凋亡率分别为(27.64±17.22)%、(33.73±14.82)%、(42.30±9.81)%和(69.27±11.50)%,均高于对照组(3.48±1.13)%(P<0.05或P<0.01);③5、10、20、40 mmol/L NAC能够引起G0/G1期细胞比例明显升高,S期比例明显降低(P值均<0.01);④5、10、20、40mmol/L NAC处理组3T3细胞Ⅰ型前胶原mRNA表达均低于对照组(P<0.05或P<0.01).结论 NAC可直接抑制小鼠肺成纤维细胞增殖,诱导其发生凋亡,并降低其胶原合成.

Efficacy of N-acetylcysteine on proliferation and collagen synthesis of mouse lung fibroblast 3T3

Objective To study the effects of N-acetylcysteine(NAC) on the proliferation,apoptosis and collagen synthesis of mouse lung fibroblast 3T3. Methods The 3T3 cell was cultured in complete medium of DMEM/F12. After different concentrations of NAC were administrated, the cell proliferations were tested by methylthiazolyhetrazolium, the apoptosis and cell cycle were detected with flow cytometer, and mRNA expression of type Ⅰ procollagen was tested with RT-PCR. Results ①The growth of 3T3 cell was significantly inhibited by NAC at different concentrations (5, 10, 20 and 40 mmol/L). ② After administration of NAC at different concentrations (5, 10, 20 and 40 mmol/L), the apoptosis rates were (27.64±17.22)%,(33.73±14.82)%, (42.30±9.81)% and (69.27±11.50)% respectively,which were higher than those of control (3.48±1.13)% (P<0.05 or P <0.01). ③ The cells proportions in G0/G1 phase were increased by NAC at different concentrations (5,10,20 and 40 mmol/L), while the changes in S-phase were significantly decreased (all P< 0.01). ④The mRNA expression of type Ⅰ procollagen in 3T3 cell was decreased after administration of NAC at 5,10,20 and 40 mmol/L respectively as compared with that of control (P <0.05 or P <0.01). Conclusions Administration of NAC can induce apoptosis, inhibit the proliferation and the collagen synthesis of 3T3 cell directly.