内源性一氧化氮合酶抑制物及其水解酶变化与子痫前期发病的关系

目的 探讨子痫前期孕妇血浆内源性一氧化氮合酶抑制物--非对称性二甲基精氨酸(ADMA)的水平变化及胎盘组织中一氧化氮合酶抑制物水解酶--二甲基精氨酸二甲胺水解酶2(DDAH-2)的表达变化与子痫前期发病的关系.方法 选择2004年1月至2005年1月在广州医学院第三附属医院住院分娩的子痫前期孕妇30例(子痫前期组),以同期正常晚期妊娠妇女10例(正常妊娠组)为对照.采用高效液相色谱(HPLC)分析法测定两组孕妇血浆ADMA水平;采用荧光定量PCR技术检测两组孕妇胎盘组织中DDAH-2 mRNA的表达.结果 (1)血浆ADMA水平:子痫前期组孕妇血浆ADMA水平为(18.0±7.2)mg/L,高于正常妊娠组的(10.3±1.7)mg/L,两组比较,差异有统计学意义(P<0.01);子痫前期组中,发病孕周<34周者血浆ADMA水平为(22.0±7.0)mg/L,显著高于发病孕周≥34周者的(12.7±2.8)ms/L,两者比较,差异也有统计学意义(P<0.01).(2)胎盘组织DDAH-2 mRNA的表达量:子痫前期组胎盘组织中DDAH-2 mRNA的表达量为1×10(5.23±0.45) copy/μ,显著低于正常妊娠组的1×10(5.65±0.08)copy/μ,两组比较,差异有统计学意义(P<0.01);子痫前期组中,发病孕周<34周者胎盘组织DDAH-2 mRNA表达量为1×10(5.02±0.46)copy/μ,显著低于发病孕周≥34周者的1 ×10(5.61±0.19)copy/μ,两者比较,差异也有统计学意义(P<0.01).结论 胎盘组织中DDAH-2 mRNA的低表达与血浆ADMA水平的升高,可能与子痫前期的发病有关.

Relationship between changes of endogenous nitric oxide synthase inhibitor and hydrolase and initiation of pre-eclampsia

Objective To investigate the role of dimethylarginine dimethylaminohydrolase-2 (DDAH-2)/asymmetric dimethylarginine(ADMA)in pathophiology of preeclampsia by detecting expression of DDAH-2 in placenta and serum plasma ADMA.Methods From Jan.2004 to Jan.2005,30 preeclampsia patients(PE group)were chosen in the Third Affiliated Hospital.Guangzhou Medical College matched with 10 normal third trimester women as control(control group).The placental DDAH-2 mRNA expression was detected by fluorescence quantitative polymerase chain reaction(FQ-PCR)and the plasma concentration of ADMA WSB determined by high performance liquid chromatography(HPLC).Results(1)The level of ADMA in PE group was significantly higher that than of control group(18.0±7.2)mg/L vs.(10.3±1.7)mg/L,P<0.01].The expression level of ADMA in preeclampsia occurring before 34 gestatinal weeks WaS significantly higher than that of preeclampsia occurring after 34 gestational weeks(22.0±7.0)ms/L vs.(12.7±2.8)mg/L,P<0.01].(2)The Placental DDAH-2 mRNA expression in preeclampsia patients was remarkably lower than that of control group1×10(5.23±0.45)copy/μlvs.1×10(5.65±0.08)copy/μl,P<0.01].The Placental DDAH-2 mRNA in preeclampsia occurring before 34 gestatinal weeks was significantly lower than that of preeclampsia occurring after 34 gestational weeks 1×10(5.02±0.46)copy/μl vs.1×10(5.61±0.19)copy/μl,P<0.01].Conclusion Our results suggested that low expression of DDAH-2 in placenta and increased serum ADMA level might confer the susceptibility to preeclampsia. lamp ia occurri