不同产地浙贝母的基因序列及生物碱含量比较

目的：探讨道地药材形成的基因基础。方法：以AS和AS－1为引物对4个不同产地浙贝母的5S－rRNA基因间区进行了PCR扩增和测序，并采用酸性染料比色法测定了它们的总生物碱含量及用柱前衍生化气相色谱法测定了其中2个单体生物碱的含量。结果：不同产地浙贝母的5S－rRNA基因间区具有相同的碱基序列，均为588bp；它们的总生物碱含量相当，气相色谱结果也表明，它们含有相同的单体生物碱，只是各单体生物碱的含量不同，结论：不同产地浙贝母的差异不是由碱基序列, 而是由小环境因素引起的。

Sequences of 5S-rRNA GeneSpacer Region and Comparison of Alkaloid Content in Fritillaria thunbergiifrom DifferentHabitats

ObjectiverTo study the genetic basis of the formation of indigenous Chinese medicine materials.Methods:The 5S-rRNA gene spacer regions in F. thunbergii from different habitats were amplified with AS and AS-1 as primers, and then sequenced. Total alkaloid contents were assayed by acid dye colorimetry, and 2 main alkaloid contents were assayed by pre-column derivatization and gas chromatographic method.Result;The sequenues of 5S-rRNA gene spacer regions in F. thunbergii from different habitats were same,and the length of them was 588 bp.They had same content total alkaloid.The results of gas chromatography showed that they had same kinds of monomer alkaloids,but the contents of different monomer alkaloids were different. Conclusion: The difference of alkaloid content in F. thunbergii from various habitats isn't resulted from base sequence variation,but from microenvironment.