黄樟素氧化物对去除成纤维细胞生长因子所诱导的血管内皮细胞凋亡及生长的影响

目的:研究黄樟素氧化物对去除成纤维细胞生长因子(FGF)诱导的血管内皮细胞凋亡及生长的影响.方法:光学显微镜观察细胞形态学变化;MTT法测定细胞生长;DNA电泳和荧光显微技术检测DNA断裂;流式细胞术测定细胞周期分布.结果:黄樟素氧化物5-25mg/L处理去除FGF的血管内皮细胞24h,细胞铺展和生长被促进,细胞脱壁和DNA片段化被抑制,黄樟素氧化物10mg/L对细胞周期分布无明显影响.黄樟素氧化物50-100 mg/L促进血管内皮细胞的脱壁和DNA片段化,黄樟素氧化物100mg/L将细胞周期阻断于G_2-M期.结论:黄樟素氧化物在10mg/L时抑制血管内皮细胞凋亡,而在100mg/L时促进其凋亡,该药物对血管内皮细胞生长和凋亡有重要影响.

Effect of safrole oxide on vascular endothelial cell growth and apoptosis induced by deprivation of fibroblast growth factor

AIM: To investigate effect of safrole oxide on cell growth and apoptosis induced by deprivation of survival factors (fibroblast growth factors, aFGF and bPGF) in vascular endothelial cells (VEC). METHODS: Morphological changes were observed by light microscopy. Cell growth was determined by MTT (3-[4, 5-dimethyl thiazol-2-yl]-2, 5-diphenyltetrazolium) method. DNA fragmentation was analyzed by agarose gel electrophoresis and fluorescence microscopy. Cell cycle distribution was analyzed by flow cytometry (FCM). RESULTS: The cells deprived of FGF were exposed to safrole oxide 5 -25 mg/L for 24 h. Cells spreading and growth were promoted (P < 0.01), detachment and DNA fragmentation of these cells were suppressed (P < 0.01), safrole oxide 10 mg/L had no obvious effect on cell cycle distribution ( P > 0.05). When the cells were treated with safrole oxide 50 - 100 mg/L, detachment and DNA fragmentation of VEC were promoted (P < 0.01). The cell cycle was blocked at G2-M phase by safrole oxide 100 mg/L. CONCLUSION: Safrole oxide 10 mg/L inhibited, but 100 mg/L promoted apoptosis of VEC. Safrole oxide might be an important compound that affects VEC growth and apoptosis.