银染PCR-SSCP法检测52例白血病患者p53基因点突变

目的通过检测白血病患者p53基因点突变，了解其与白血病的发展、治疗及预后的关系。方法用银染PCR-SSCP法检测52例不同类型白血病p53基因第5、6、7、8外显子的点突变。结果在45例急性白血病患者中有5例发生p53基因突变，其中3例对化疗不敏感；6例慢性粒细胞白血病中1例有p53基因突变，此例患者在住院1个月内死亡，1例毛细胞白血病发生p53基因点突变。结论p53基因点突变的检测对指导临床治疗及判断预后有一定价值。

Mutation of the p53 in human leukemia detected by sliver staining PCR-SSCP

Objective To investigate the relationship between the mutation of p53 gene and the carcinogenesis,development and prognosis of patients with leukmia.Methods We examined the mutation of p53 gene in exon 5,6,7,8 in 52 patients with leukemia by silver staining PCR SSCP.Results Mutation of p53 were found in 7 of 52 patients:mutation of p53 gene were found in 5 of 45 acute leukemia patients,among them three patients showed a poor response to chemical therapy;In 6 chronic myelocytic leukemia patients one was found the mutation of p53,she was died after one month since she had entered the hospital;one hairy cell leukemia patient was found the mutation of p53.Conclusion The mutation of p53 gene is prognostic factor for survival,it is instructive for clinical therapy.