上调SIRT1减少同型半胱氨酸诱导的血管内皮细胞凋亡

目的 研究同型半胱氨酸处理对血管内皮细胞中SIRT1表达的影响,并探讨上调血管内皮细胞中SIRT1表达对同型半胱氨酸诱导的血管内皮细胞凋亡的影响.方法 建立同型半胱氨酸诱导的血管内皮细胞凋亡模型,通过CCK8试剂盒检测细胞活性,通过流式细胞仪检测细胞凋亡,通过qPCR法检测mRNA表达和通过免疫印记法检测蛋白表达.结果...

Up-regulation of SIRT1 reduces homocysteine-induced the apoptosis of vascular endothelial cells

Objective To study the effect of homocysteine on SIRT1 expression in vascular endothelial cells,and explore the effect of up-regulation of SIRT1 on Hcy-induced apoptosis in vascular endothelial cells.Methods The cell activity was detected by CCK8 kit,the apoptosis was detected by flow cytometry,the mRNA expression was detected by qPCR,and the protein expression was detected by Western blot.Results Homocysteine induced apoptosis of vascular endothelial cells and inhibited SIRT1 expression in a dose-dependent manner.Up regulation of SIRT1 expression in vascular endothelial cells not only significantly reduced homocysteine induced apoptosis of vascular endothelial cells(P<0.001),but also significantly inhibited the expression of Pro apoptotic gene BCL2(P<0.001)and significantly promoted the expression of anti apoptotic genes Bax and Cyt C(P<0.001).Conclusion Homocysteine inhibits the expression of SIRT1 in vascular endothelial cells,and up-regulation of SIRT1 expression can reduce homocysteine-induced apoptosis of vascular endothelial cells.