杀虫磺原药大鼠亚慢性毒性研究

目的研究杀虫磺原药对大鼠亚慢性经口毒性,探讨其亚慢性毒性的阈作用剂量和最大无作用剂量。方法雌鼠剂量以135、67.50、22.50mg/kg,雄鼠剂量以157.5、78.752、6.25mg/kg,连续90d给予杀虫磺原药,观察动物的一般状况,体重增长及血液、生化指标并解剖进行病理检查。结果杀虫磺原药高剂量组动物,染毒8周后出现被毛蓬松、活动减少、体重减轻等症状;染毒结束后血清中谷草转氨酶（AST）升高,肝及雄鼠肾脏器系数增大,组织病理学检查显示：肝细胞明显浊肿,胞浆疏松,汇管区可见慢性炎细胞浸润。中剂量组动物染毒结束后谷草转氨酶（AST）显著升高,雄鼠肾脏器系数增大;肝细胞病变相同,但程度较轻。低剂量组动物一般行为、体重增长、尿常规、血液学、血液生化学、脏器重量、脏器系数及病理学检查均未见变化。结论本实验条件下,初步确定大鼠90d亚慢性经口最大无作用剂量,雌性为22.50mg/kg;雄性为26.25mg/kg。

Study on Subchronic Toxicity of Bensultap to Rats

Objectives To study the subchronic toxicity of bensultap to rats and to provide the toxicity mechanism,threshold dose and its NOAEL(no observed adverse effect level). Methods The rats were gavaged with bensultap at the dose of 135.0,67.50,22.50 mg/kg(female rats) and 157.5,78.75,26.25 mg/kg(male rats) once a day for 90 days.General conditions were observed,weight gains were checked,Blood routine and biochemical indicators were tested.Tissue samples were examined. Results 8 weeks after intoxication,the rats in high dose group showed hair fluffy,less activity,weight loss.Aspartate aminotransferase(AST) increased.Liver and kidney index(male rats) also increased.Histopathologic examination revealed that hepatocytes appeared cloudy swelling and hyalomitome appeared porous,portal tract had inflammatory cells infiltration.In medium dose group,AST increased significantly,kidney index increased in male rats,and hepatocytes appeared the same as the high dose group.No significant difference was found in low dose group. Conclusion Bensultap has toxic effect on liver and kidney.NOAEL is 22.50 mg/kg(female rats) and 25.25 mg/kg(male rats).