| 海马区Bcl-2蛋白在新生大鼠脑缺血后的表达 |
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| 引用本文： | 萨丽波,李宇. 海马区Bcl-2蛋白在新生大鼠脑缺血后的表达[J]. 沈阳医学院学报, 2011, 13(1): 16-18 |
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| 作者姓名： | 萨丽波 李宇 |
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| 作者单位： | 1. 沈阳医学院基础医学院生理学教研室,辽宁,沈阳,110034
2. 辽宁省金秋医院特诊一病房 |
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| 摘 要： | 目的：通过研究新生大鼠脑缺血时Bcl-2蛋白在海马区的表达特点,探讨Bcl-2蛋白在缺血缺氧性脑损伤的作用。方法：48只7日龄新生SD大鼠随机分为1个对照组和7个实验组。给动物吸入含有92%氮气和8%氧气混合气体建立新生大鼠缺氧缺血性脑损伤（HIBD）动物模型,分别在脑损伤后不同时间点（0.5、1、3、6、12、24、48、72 h等）断头处死动物,取海马组织,用免疫组织化学方法检测海马脑神经细胞凋亡及Bcl-2蛋白表达情况。结果：TUNEL染色的阳性细胞数与Bcl-2蛋白阳性的细胞数在HIBD后的不同时间点出现分别有显著差异,海马区Bcl-2蛋白阳性细胞在HIBD后立即出现,6 h达到高峰。结论：Bcl-2蛋白强表达于海马缺血区和缺血周边区的神经元,与神经元的存活或死亡有一定联系。
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| 关 键 词： | Bcl-2蛋白 新生大鼠 缺氧缺血性脑损伤 |
The Expression of Bcl-2 Protein after Ischemic Brain Damage in Neonatal Rat |
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| SA Li-bo,LI Yu. The Expression of Bcl-2 Protein after Ischemic Brain Damage in Neonatal Rat[J]. Journal of Shenyang Medical College, 2011, 13(1): 16-18 |
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| Authors: | SA Li-bo LI Yu |
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| Affiliation: | 1.Department of Physiology,Shengyang Medical College,Shengyang 110034,China;2.Ward One of Jinqiu Hospital of Liaoning Province) |
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| Abstract: | Objective:To study the effect of Bcl-2 protein in cell apoptosis by observing the expression of Bcl-2 protein in hippocampus of hypoxic ischemic brain damage(HIBD).Methods:48 neonatal rats were used in isham operated group and 7 trial groups.The models of HIBD were established in neonatal rats by inhaling the mixed gases of 92%N2 and 8%O2,and the animals were sacrificed by dislocation their heads at different time morphological analysis.Results:The positive cell of Bcl-2 protein increased after HIBD and reached the peak at 6h and then decreased gradually following HIBD.Conclusions:The expression of Bcl-2 protein in hippocampus is stronger than other areas of brain,and there is relationship between cell apoptosis and Bcl-2 protein. |
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| Keywords: | Bcl-2 protein neonatal rat hypoxic ischemic brain damage |
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