连接蛋白43、Kai1和PTEN蛋白在皮肤基底细胞癌中的表达及意义

 目的 探讨皮肤基底细胞癌（basal cell carcinoma，BCC）发生发展及其特殊生物学行为的相关因素。方法 免疫组织化学SP法分别检测35例皮肤BCC、10例正常皮肤和18例皮肤鳞状细胞癌（squamous cell cacinoma，SCC）组织中Cx43、Kai1、PTEN蛋白的表达；核酸分子原位杂交法检测Cx43 mRNA的表达。应用计算机图像分析系统分别检测各组中各种检测指标的阳性面积和表达强度； SPSS（13.0）软件包进行统计分析。结果 （1）Cx43蛋白、Cx43 mRNA在皮肤表皮中的表达呈强阳性，在BCC组呈阳性或弱阳性，在SCC组呈阴性或弱阳性。Cx43及其 mRNA在BCC组的表达与正常皮肤组及SCC组比较，差异均有统计学意义（P<0.01）。（2）Kai1、PTEN蛋白在皮肤表皮中的表达呈强阳性，在BCC组呈阳性或弱阳性，在SCC组呈阴性或弱阳性。Kai1、PTEN蛋白在BCC组的表达与正常皮肤组及SCC组比较，差异均有统计学意义（P<0.05）。（3）相关分析显示，BCC组织中，PTEN蛋白的表达与Kai1蛋白呈正相关(r=0.629，P<0.01)，与Cx43蛋白(r=0.519，P＜0.01)也呈正相关；Cx43蛋白的表达与Cx43 mRNA呈正相关(r=0.732，P<0.01)。结论 （1）与正常皮肤组织比较，BCC中Cx43、Kai1、PTEN蛋白和Cx43mRNA低表达，可能在BCC发生发展的过程中发挥重要作用；（2）与SCC组织相比较，BCC中Cx43、Kai1、PTEN蛋白和Cx43mRNA高表达，可能与BCC生长缓慢及极少转移的生物学特性有关。

Expression and Significance of Connexin43,Kai1 and PTEN in Skin Basal Cell Carcinoma

Objective To investigate the occurrence，development and special biological behavior of skin basal cell carcinoma(BCC).Methods The expressions of connexin43，Kai1 and PTEN proteins were detected by immunohistochemical S P method in 35 patients with BCC，10 case with normal tissues and 18 patients with squamous cell carcinoma(SCC)；And the expressions of Cx43 mRNA were detected by in situ hybridization.The expression levels (positive area and optical density)of connexin43，Kai1 and PTEN proteins and Cx43 mRNA were detected respectively by computerized image analysis in normal skin, BCC and SCC.All the data were input into the computer and statistically analyzed with SPSS(13.0) software.Results(1) Cx43 protein and mRNA showed strongly positive expression in skin epidermis tissues，positive or weakly positive expression in BCC group，and negative or weak positive in SCC group. Compared with normal skin group and SCC group，the expressions（positive area and optical density）of Cx43 protein and mRNA in BCC group were significant increased（P<0.01）；(2) Kai1 and PTEN protein showed strongly positive expression in skin epidermis tissues，positive or weakly positive expression in BCC group，and negative or weak positive in SCC group. Compared with normal skin group and SCC group，the expression differences of Kai1，PTEN protein in BCC group all had significantincreased（P<0.05）；(3) There was a positive correlation between the expressions of PTEN and Kai1 (r=0.629，P<0.01)， PTEN and Cx43 (r=0.519，P<0.01)，as well as Cx43 protein and its mRNA(r=0.732，P<0.01) in BCC.Conclusions(1) The lower expression of Cx43, Kai1，PTEN proteins and Cx43 mRNA might be play important roles in BCC occurrence and development. (2)The higher expression of Cx43，Kai1，PTEN proteins and Cx43 mRNA in BCC was related to the biological characteristics such as slow growth and rare metastasis.