党参多糖对环磷酰胺所致小鼠贫血的治疗效果观察

目的 探讨党参多糖对环磷酰胺(CTX)所致小鼠贫血的疗效.方法 将60只SPF级健康雄性BALB/c小鼠均分为空白组、模型组和低、中、高剂量组及阳性对照组6组,除空白组外,其他5组分别于实验第1天按100 mg/kg剂量腹腔注射CTX,每日1次,空白组予等容积生理盐水腹腔注射,连续7 d.实验第2天注射CTX后2 h,...

Therapeutic Effectiveness of Codonopsis Pilosula Polysaccharide on Mice with Anemia Induced by Cyclophosphamide

Objective To investigate the therapeutic effectiveness of Codonopsis Pilosula Polysaccharide on mice with anemia induced by Cyclophosphamide(CTX).Methods A total of 60 SPF healthy male BALB/c mice were equally divided into 6 groups blank group,model group,low,medium and high dose groups and positive control group.Except for blank group,the other 5 groups were intraperitoneally injected with CTX by the dose of 100 mg/kg on the first day of the experiment for once a day.The blank group was intraperitoneally injected with the equal volume of physiological saline for 7 d continuously.At 2 h after CTX injection on the second day of the experiment beginning,the low,medium and high dose groups were given 10,20 and 40 ml/kg Codonopsis Pilosula Polysaccharide by gavage respectively,while positive control group was given 400 mg/kg Shengxuening Solution by gavage once a day for 7 d continuously.Levels of red blood cells(RBC),hemoglobin(Hb)and hematocrit(HCT)were detected by automatic blood cell counter,and levels of erythropoietin(EPO)and multi colony stimulating factor(multi-CSF)were detected by enzyme linked immunosorbent assay(ELISA),and levels of CD71+and Ter119+in peripheral blood were detected by flow cytometry in each group.Results RBC,Hb and HCT levels in peripheral blood in model group were significantly lower than those in blank group(P<0.05).RBC,Hb and HCT levels in low,medium and high dose groups were significantly increased compared with those in model group(P<0.05);RBC,Hb and HCT levels in low and medium dose groups were significantly lower than those in positive control group(P<0.01).Levels of multi-CSF and EPO in peripheral blood in model group were significantly lower than those in blank group(P<0.05).Levels of multi-CSF and EPO in low,medium and high dose groups were significantly increased compared with those in model group(P<0.05).The multi-CSF levels in low,medium and high dose groups were significantly lower than that in positive control group(P<0.01),and multi-CSF level was increased with increasing intervention dose.EPO levels in low and medium dose groups were significantly lower than that in positive control group(P<0.01).Levels of CD71+and Ter119+in model group were significantly lower than those in blank group(P<0.01).Levels of CD71+and Ter119+in low,medium and high dose groups were significantly increased compared with those in model group(P<0.01).Levels of CD71+and Ter119+in low and medium dose groups were significantly lower than those in positive control group(P<0.01).Conclusion Codonopsis Pilosula Polysaccharide may effectively improve mice with anemia induced by CTX.