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白芍总苷通过miR-23b-3p/FoxA1对肺炎链球菌诱导的肺泡上皮细胞凋亡的影响
引用本文:李玉磊,张瑞芳,刘莉敏. 白芍总苷通过miR-23b-3p/FoxA1对肺炎链球菌诱导的肺泡上皮细胞凋亡的影响[J]. 临床肺科杂志, 2022, 27(2): 202-208. DOI: 10.3969/j.issn.1009-6663.2022.02.009
作者姓名:李玉磊  张瑞芳  刘莉敏
作者单位:430064 湖北 武汉,武汉科技大学附属天佑医院 呼吸与危重症医学科
摘    要:目的 探讨白芍总苷及对肺炎链球菌诱导的肺泡上皮细胞凋亡的影响分子机制.方法 肺泡上皮细胞A549随机分为对照(Con)组、模型(Model)组(1×108 CFU/mL的肺炎链球菌感染)、Model+白芍总苷低、中、高剂量组(17.5 mg/mL、35 mg/mL、70 mg/mL的白芍总苷+1×108 CFU/mL的...

关 键 词:白芍总苷  miR-23b-3p  肺炎链球菌  肺泡上皮细胞  凋亡  炎症

Effect of total glucosides of paeony on alveolar epithelial cell apoptosis induced by Streptococcus pneumoniae via miR-23b-3p/FoxA1
LI Yu-lei,ZHANG Rui-fang,LIU Li-min. Effect of total glucosides of paeony on alveolar epithelial cell apoptosis induced by Streptococcus pneumoniae via miR-23b-3p/FoxA1[J]. Journal of Clinical Pulmonary Medicine, 2022, 27(2): 202-208. DOI: 10.3969/j.issn.1009-6663.2022.02.009
Authors:LI Yu-lei  ZHANG Rui-fang  LIU Li-min
Affiliation:(Department of Respiratory and Critical Care Medicine,Tianyou Hospital Affiliated to Wuhan University of Science and Technology,Wuhan,Hubei 430064,China)
Abstract:Objective To explore the total glucosides of paeony and the molecular mechanism of its effect on the apoptosis of alveolar epithelial cells induced by Streptococcus pneumoniae.Methods Alveolar epithelial cells A549 were randomly divided into the control group,the model group(1×108CFU/mL of Streptococcus pneumoniae infection),the model+total glucosides of paeony low,medium,and high dose groups(17.5 mg/mL,35 mg/mL,70 mg/mL total glucosides of paeony+1×108CFU/mL of Streptococcus pneumoniae infection),the model+total glucosides of paeony+anti-miR-23 b-3 p group(transfected with miR-23 b-3 p inhibitor+70 mg/mL total glucosides of paeony+1×108CFU/mL Streptococcus pneumoniae infection).It used cell counting kit 8(CCK-8)to detect cell viability,flow cytometry to detect cell apoptosis,enzyme-linked immunosorbent assay(ELISA)to detect the levels of IL-6 and IL-10,real-time fluorescent quantitative PCR(RT-qPCR)to detect the expression level of miR-23 b-3 p,Western blotting to detect the expression of FoxA1 protein,and dual luciferase reporter experiment to detect targeting relationship of miR-23 b-3 p and FoxA1.Results After treatment with low,medium and high doses of total glucosides of paeony,the activity of alveolar epithelial cells induced by Streptococcus pneumoniae was increased,the rate of apoptosis was decreased,the content of IL-6 was decreased,the content of IL-10 was increased,the expression of miR-23 b-3 p was increased,and the FoxA1 protein expression was decreased,in a dose-dependent manner(P<0.05).After over-expression of miR-23 b-3 p,FoxA1 protein expression was decreased,cell viability was increased,cell apoptosis rate was decreased,IL-6 content was decreased,and IL-10 content was increased(P<0.05).The inhibition of miR-23 b-3 p could reverse the effect of total glucosides of paeony on A549 cell apoptosis,activity and IL-6 and IL-10 content induced by Streptococcus pneumoniae.miR-23 b-3 p targeted Fox A1.Conclusion The total glucosides of paeony can inhibit the apoptosis and inflammation of alveolar epithelial cells induced by Streptococcus pneumoniae by regulating the miR-23 b-3 p/Fox A1 axis.
Keywords:total glucosides of paeony  miR-23b-3p  Streptococcus pneumoniae  alveolar epithelial cells  apoptosis  inflammation
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