银杏叶中银杏内酯A分离纯化工艺研究

目的:建立一种简易的白银杏叶中提取、分离和纯化银杏内酯A的方法.方法:依次通过乙醇溶液搅拌提取、乙酸乙酯萃取、大孔树脂吸附与洗脱及结晶和重结晶对银杏叶中的银杏内酯A进行分离纯化.以银杏内酯A的得率和纯度为指标,对银杏内酯A的分离纯化工艺进行优选.结果:确定银杏内酯A提取、分离和纯化的最佳工艺条件为:将银杏叶乙醇提取物混...

Study on Isolation and Purification of Ginkgolide A from Ginkgo Leaves

Objective：To establish a simple method of extraction, isolation and purification for ginkgolide A from ginkgo leaves. Methods： The ginkgolide A in ginkgo leaves was extracted, isolated and purified by extracting with alcohol-water solution and ethyl acetate, by macroporous resin＇s adsorption and elution, as well as by crystallization and recrystallization. The optimum conditions of the process were studied by taking the yield and purity of ginkgolide A as index. Results： The optimum conditions of the process were con~ firmed as follows： the pH value of ethanol extract from ginkgo leaves was adjusted to 3 before extraction with ethyl acetate; the pH value of ethyl acetate extract was adjusted to 3 and then NM100 macroporous resin was used to adsorb the ethyl acetate extract, with the flow rate of 1 mL·min^-1; The column was eluted with 30 % and 50 % ethanol respectively, and then the 50 % ethanol eluant was collected and concentrated; The 50 % ethanol eluant was crystallized at 4 ℃, and then the macrocrystal was recrystallized in ethanol- water system with the ratio of 2： 1. The purity of the ginkgolide A prepared in this process was above 99 %, with the yield of 60 %. Conclusion： The process is stable and feasible. It can be applied to industrial production.