| 5-氟尿嘧啶对绒癌JAR细胞凋亡的作用机制 |
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| 引用本文： | 车小群,李英勇,练晓勤,杨爱莲,温宝宁.5-氟尿嘧啶对绒癌JAR细胞凋亡的作用机制[J].中国妇幼保健,2011,26(11):1705-1709. |
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| 作者姓名： | 车小群 李英勇 练晓勤 杨爱莲 温宝宁 |
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| 作者单位： | 1. 广东省江门市中心医院妇科,529030
2. 广东医学院附属医院妇产科 |
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| 基金项目： | 广东省医学科研基金立项项目 |
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| 摘 要： | 目的:探讨5-氟尿嘧啶(5-fluorouracil,5-FU)对人类绒毛膜癌细胞株JAR细胞凋亡的作用机制。从分子水平分析绒癌细胞凋亡及凋亡抑制基因XIAP在此凋亡过程中的作用。方法:RT-PCR法检测5-FU对JAR细胞XIAP mRNA表达的影响。结果:5-FU在0～40μg/ml范围内可以呈剂量依赖性下调XIAP基因mRNA的表达,在40～80μg/ml范围内,随着5-FU剂量的增加,下调能力有所减弱,XIAP的表达反而有所增强。结论:5-FU诱导绒癌细胞凋亡可能是其抗肿瘤作用机制之一,5-FU诱导细胞凋亡的机制可能与XIAP mRNA的表达有关。
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| 关 键 词： | 5-氟尿嘧啶 绒癌 XIAP |
The mechanism of 5 - fluorouracil on apoptosis of choriocarcinoma JAR cells |
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| CHE Xiao-Qun,LI Ying-Yong,LIAN Xiao-Qin et al.The mechanism of 5 - fluorouracil on apoptosis of choriocarcinoma JAR cells[J].Maternal and Child Health Care of China,2011,26(11):1705-1709. |
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| Authors: | CHE Xiao-Qun LI Ying-Yong LIAN Xiao-Qin |
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| Institution: | CHE Xiao-Qun,LI Ying-Yong,LIAN Xiao-Qin et al.Department of Gynecology,Central Hospital of Jiangmen,Jiangmen 529030,Guangdong,China |
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| Abstract: | Objective:To explore the mechanism of 5-fluorouracil on apoptosis of choriocarcinoma JAR cells,analyze the effects of apoptosis of choriocarcinoma cells and inhibitor of apoptosis gene-XIAP in the course of apoptosis of choriocarcinoma JAR cells at the molecular level. Methods:RT-PCR was used to detect the effect of 5-fluorouracil on XIAP mRNA expression in JAR cells. Results:5-fluorouracil within the range of 0～40 μg/ml down-regulated the expression of XIAP mRNA,showing a dose-dependent manner;within the range of 40～80 μg/ml,with the increase of 5-fluorouracil dose,the down-regulation ability decreased,but the expression of XIAP mRNA increased. Conclusion:The apoptosis of choriocarcinoma JAR cells induced by 5-fluorouracil may be one of the anti-tumor mechanisms,the mechanism may be related to the expression of XIAP mRNA. |
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| Keywords: | XIAP |
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