黄连解毒汤对自发性高血压大鼠血压及其内皮祖细胞NO分泌功能的影响

目的:观察黄连解毒汤(HLJDT)对自发性高血压大鼠(SHR)血压及其内皮祖细胞(EPCs)一氧化氮(NO)分泌功能的影响,并探讨其作用机制。方法:60只雄性12周龄SHR随机分为5组,分别为模型组,卡托普利阳性药组,HLJDT低、中、高剂量组,每组12只,12只同周龄雄性WKY大鼠作为正常组。正常组和模型组予生理盐水ig(20 m L·kg~(-1)),其余各组分别给予卡托普利ig(1.35 mg·kg~(-1)),HLJDT低剂量ig(13.5 mg·kg~(-1)),HLJDT中剂量ig(27 mg·kg~(-1)),HLJDT高剂量ig(54mg·kg~(-1)),均1次/d,连续2周。用药前及预处理结束后当天采用BP-6大鼠无创血压测试仪测定各组大鼠尾动脉收缩压,采用密度梯度离心法分离培养骨髓来源的EPCs,经Dil-ac-LDL和FITC-UEA-I双染色鉴定后,分别采用细胞计数试剂盒(CCK-8)检测法、迁移小室(Transwell)小室、黏附实验及NO检测分析各组EPCs增殖、迁移、黏附能力及NO分泌功能。结果:与正常组比较,模型组SHR血压明显升高,SHR内皮祖细胞数量,增殖能力,迁移能力,黏附能力及NO分泌功能明显降低(P0.01);与模型组比较,HLJDT可以降低SHR血压,HLJDT低、中、高剂量组SHR内皮祖细胞数量,增殖能力,迁移能力,黏附能力及NO分泌功能均有增加(P0.05,P0.01)。结论:HLJDT能降低SHR血压,并且能增加EPCs的数量,提高EPCs的增殖、迁移、黏附能力及NO分泌功能。

Influence of Huanglian Jiedu Tang on Function of NO Secretion in Endothelial Progenitor Cells and Blood Pressure in Spontaneous Hypertension Rats

Objective: To observe the effects of Huanglian Jiedu Tang (HLJDT) on function of NO secretion of endothelial progenitor cells (EPCs) and blood pressure in spontaneous hypertension rats (SHR). Method: The 60 male 12-week-old SHR were randomly divided into five groups:model control group, captopril positive control group, HLJDT low-dose group, HLJDT middle-dose group, HLJDT high-dose group, with 12 rats in each group. Another 12 male Wistar-Kyoto(WKY) rats were used as blank group. WKY control group and model group were given normal saline ig(20 mL·kg^-1), while the remaining groups were respectively given captopril ig(0.001 35 g·kg^-1), HLJDT low-dose ig(0.0135 g·kg^-1), HLJDT middle-dose ig(0.027 g·kg^-1), HLJDT high-doseig(0.054 g·kg^-1) for 2 weeks. On the day before the administration and after the pretreatment, BP-6 rat noninvasive blood pressure tester was used to determine each group''s caudal artery systolic blood pressure; the density gradient centrifugation separation method was adopted to isolate and culture endothelial progenitor cells from bone marrows. The cultured EPCs were identified by Dil-ac-LDL and FITC-UEA-I double-staining, and then assayed with CCK-8, Transwell chamber and adhesion function test and NO test, in order to analyze EPC proliferation, migration, adhesion capacity and NO secretion of each group. Result: Compared with the normal group, the model group showed significant increase in SHR blood pressure, and significant decrease in the number of EPCs, proliferation, migration capacity, adhesion capacity and NO secretion (P<0.01); compared with the model group, HLJDT can decrease the SHR blood pressure, and the number of EPCs, proliferation, migration capacity, adhesion capacity and NO secretion were increased in all of HLJDT groups (P<0.05, P<0.01). Conclusion: HLJDT can decrease SHR blood pressure, and increase the number of EPCs, and EPCs'' proliferation, migration capacity, adhesion capacity and NO secretion.