| 高转移胃癌细胞株的建立及其肿瘤干细胞的生物学特征 |
| |
| 引用本文： | 遇珑,舒雄,刘辉琦,刘军,孙力超,孙立新,杨治华,冉宇靓.高转移胃癌细胞株的建立及其肿瘤干细胞的生物学特征[J].中国肿瘤生物治疗杂志,2016,23(4):486-491. |
| |
| 作者姓名： | 遇珑 舒雄 刘辉琦 刘军 孙力超 孙立新 杨治华 冉宇靓 |
| |
| 作者单位： | 国家癌症中心/中国医学科学院北京协和医学院 肿瘤医院,细胞及分子生物学实验室,北京100021 |
| |
| 基金项目： | 国家自然科学基金（No. 31371445）; 国家高技术研究发展计划（863计划）(No. 2014AA020537) |
| |
| 摘 要： | 目的:建立高转移胃癌细胞株并研究其肿瘤干细胞生物学特征.方法:将胃癌细胞株SNU-5接种至裸鼠皮下成瘤后,取肺部转移灶,通过机械分离法并反复接种裸鼠,获取细胞株SNU-5-V12,采用无血清悬浮培养及PKH26染色确定SNU-5-V12细胞中存在肿瘤干细胞.流式细胞术分析SNU-5和SNU-5-V12细胞中CD44肿瘤干细胞标志物表达情况,分选SNU-5和SNU-5-V12细胞中CD44+细胞并进行体外生物学特征研究及裸鼠致瘤实验.结果:建立高转移SNU-5-V12细胞株,SNU-5-V12细胞无血清悬浮培养11d后形成的细胞球体中存在单个PKH26阳性细胞.流式分析显示高转移SNU-5-V12细胞中干细胞标志物CD44比例显著高于SNU-5细胞(72.9±1.5)％ vs (8.96±1.2)％],且SNU-5-V12的CD44+细胞比SNU-5的CD44+细胞具有更强的自我更新能力成球率(27.8±1.7)％ vs (20.4±1.0)％,P＜0.01],转移细胞数增加1.64倍(329.5±7.5) vs (200±2.0)个,P＜0.01],耐药能力也显著增强IC50:(0.286 vs 0.196)μ∥ml,P＜0.01],裸鼠皮下接种2×102个CD44+ SNU-5-V12细胞2个月2/6裸鼠可致瘤,而CD44+ SNU-5细胞需要接种2×104个细胞2个月时仅1/6裸鼠致瘤.结论:获得高转移胃癌细胞株SNU-5-V12,其CD44+细胞体内外功能显著增强,为胃癌干细胞的靶向治疗提供有价值的细胞模型.
|
| 关 键 词： | 胃癌 肿瘤干细胞 转移 CD44 |
| 收稿时间： | 2/3/2016 12:00:00 AM |
| 修稿时间： | 5/4/2016 12:00:00 AM |
Establishment of a gastric cancer cell line with high metastasis and biological characteristics of cancer stem cell of the cell line |
| |
| YU Long,SHU Xiong,LIU Huiqi,LIU Jun,SUN Lichao,SUN Lixin,YANG Zhihua and RAN Yuliang.Establishment of a gastric cancer cell line with high metastasis and biological characteristics of cancer stem cell of the cell line[J].Chinese Journal of Cancer Biotherapy,2016,23(4):486-491. |
| |
| Authors: | YU Long SHU Xiong LIU Huiqi LIU Jun SUN Lichao SUN Lixin YANG Zhihua and RAN Yuliang |
| |
| Institution: | Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China,Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China,Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China,Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China,Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China,Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China,Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China and Laboratory of Cell and Molecular Biology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China |
| |
| Abstract: | |
| |
| Keywords: | gastric cancer cancer stem cell metastasis CD44 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国肿瘤生物治疗杂志》浏览原始摘要信息 |
| 点击此处可从《中国肿瘤生物治疗杂志》下载免费的PDF全文 |
|
